Active Recombinant Human OTUD5, GST-tagged

• Cat.No.: OTUD5-165H
• Product Overview: Recombinant human OTUD5 (amino acid residues 1-571), fused with N-terminal GST, was expressed in E.coli.

Specification

• Description: OTUD5 is a cysteine protease and is a member of the OTU (ovarian tumour) superfamily of proteins (Balakirev et al., 2003). Cloning of the human gene was first described by Kayagaki et al. (2007). Ovarian tumour family DUBs contain a papain-like catalytic core of ~180 amino acids. In addition to their catalytic domain, many OTU members have additional ubiquitin-binding domains (UBDs).
• Source: E. coli
• Species: Human
• Tag: GST
• Form: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
• Bio-activity: Deubiquitylase Enzyme Assay: The activity of GST-OTUD5 was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of GST-OTUD5 was compared confirming the deubiquitylating activity of GST-OTUD5.
• Molecular Mass: 87.7 kDa
• Purity: >51% by SDS-PAGE
• Storage: 12 months at -70°C. Avoid multiple freeze/thaw cycles.
• Concentration: 0.5 mg/ml

Gene Information

• Gene Name: OTUD5 OTU domain containing 5 [ Homo sapiens ]
• Official Symbol: OTUD5
• Synonyms: DUBA
• Gene ID: 55593
• mRNA Refseq: NM_017602.3
• Protein Refseq: NP_060072.1
• MIM: 300713
• UniProt ID: Q96G74
• Chromosome Location: Xp11.23
• Pathway: Immune System, organism-specific biosystem;Innate Immune System, organism-specific biosystem;Negative regulators of RIG-I/MDA5 signaling, organism-specific biosystem;RIG-I-like receptor signaling pathway, organism-specific biosystem;RIG-I-like receptor signaling pathway, conserved biosystem;RIG-I/MDA5 mediated induction of IFN-alpha/beta pathways, organism-specific biosystem
• Function: ubiquitin-specific protease activity