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Recombinant Human ISG15

Cat.No.: ISG15-191H
Product Overview: Recombinant human ISG15 (amino acid residues 1-157) was expressed in E.coli.
Description: ISG15 functions in various biological pathways from pregnancy to innate immune responses (Ritchie and Zhang. 2004; Zhao et al., 2004). Secretion of ISG15 from monocytes in response to type I Interferons has been shown to cause natural killer (NK) cell proliferation and an increase in non-(major histocompatibility complex) MHC cytotoxicity (Meraro et al., 2002). ISG15 has also been shown to function intracellularly, being covalently conjugated to other proteins by UBA7, UBE2L6 and E3 ligases via a multistep process analogous to ubiquitylation (Loeb and Haas. 1992; Zhao et al., 2005).
Source: E. coli
Species: Human
Form: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
Bio-activity: E1 Thioester ISG15 Loading Assay: The activity of ISG15 was validated by loading ISG15 onto the active cysteine of His-UBA7. Incubation of the His-UBA7 enzyme in the presence of ISG15 and ATP at 30?C was compared at two time points, T0 and T10 minutes. Sensitivity of the ISG15/His-UBA7 thioester bond to the reducing agent DTT was confirmed.
Molecular Mass: ~17.59 kDa
Purity: >80% by SDS-PAGE
Storage: 12 months at -70°C. Avoid multiple freeze/thaw cycles.
Concentration: 1 mg/ml
Gene Name: ISG15 ISG15 ubiquitin-like modifier [ Homo sapiens ]
Official Symbol: ISG15
Synonyms: ISG15; ISG15 ubiquitin-like modifier; G1P2, interferon, alpha inducible protein (clone IFI 15K); ubiquitin-like protein ISG15; IFI15; UCRP; ubiquitin cross-reactive protein; interferon-induced 15 kDa protein; interferon-induced 17 kDa protein; interferon-stimulated protein, 15 kDa; interferon-induced 17-kDa/15-kDa protein; interferon, alpha-inducible protein (clone IFI-15K); G1P2; IP17; hUCRP;
Gene ID: 9636
mRNA Refseq: NM_005101
Protein Refseq: NP_005092
MIM: 147571
UniProt ID: P05161
Chromosome Location: 1p36.33
Pathway: Antiviral mechanism by IFN-stimulated genes, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; ISG15 antiviral mechanism, organism-specific biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem; Interferon Signaling, organism-specific biosystem; Interferon alpha/beta signaling, organism-specific biosystem;
Function: protein tag;

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