Cat. No. : |
Adipoq-75M |
Product Overview : |
Recombinant mouse globular domain of adiponectin/acrp30 (amino acid residues, 111-247, gAcrp30) was overexpressed in E.coli and purified by using conventional chromatography techniques, 16 kDa (138 aa). |
Description : |
Adiponectin/Acrp30 (247amino acids) is adipocyte complement-related protein of 30kDa and exclusively expressed in differentiated adipocytes. Adiponectin (Acrp30) is a member of the complement factor C1q family and consists of signal sequence, Non-homologous sequence, collagen domain and globular domain (gAcrp30). Adiponectin (Acrp30) expression is reduced in a variety of obese and insulin-resistant states in human, monkeys and mice. Injection of Acrp30 (247aa) or gAcrp30 (globular domain) lowers serum glucose and free fatty acid level in mice. |
Sequences of amino acids : |
MAYMYRSAFS VGLETRVTVP NVPIRFTKIF YNQQNHYDGS TGKFYCNIPG LYYFSYHITV YMKDVKVSLF KKDKAVLFTY DQYQEKNVDQ ASGSVLLHLE VGDQVWLQVY GDGDHNGLYA DNVNDSTFTG FLLYHDTN |
Formulation : |
Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing 50 mM NaCl, 5 mM DTT, 10% glycerol |
Purity : |
> 95% by SDS - PAGE |
Concentration : |
1 mg/ml (determined by Bradford assay) |
Storage : |
Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |