Recombinant Human Adenylate Kinase 2, His-tagged

• Cat.No.: AK2-117H
• Product Overview: Recombinant human AK2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques, 28.6 kDa (259 aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Adenylate kinase (AK; adenosine triphosphate-adenosine monophosphate [ATP-AMP] phospho-transferase, EC 2.7.4.3) is a ubiquitous monomeric enzyme involved energy metabolism of prokaryotic and eukaryotic cells. Three isozymes (AK1, AK2 and AK3) are characterized in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. AK2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis.
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MAPSVPAAEP EYPKGIRAVL LGPPGAGKGT QAPRLAENFC VCHLATGDML RAMVASGSEL GKKLKATMDA GKLVSDEMVV ELIEKNLETP LCKNGFLLDG FPRTVRQAEM LDDLMEKRKE KLDSVIEFSI PDSLLIRRIT GRLIHPKSGR SYHEEFNPPK EPMKDDITGE PLIRRSDDNE KALKIRLQAY HTQTTPLIEY YRKRGIHSAI DASQTPDVVF ASILAAFSKA TCKDLVMFI
• Formulation: Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing 5 mM DTT, 20% glycerol
• Purity: > 95% by SDS - PAGE
• Concentration: 1 mg/ml (determined by Bradford assay)
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: AK2 adenylate kinase 2 [ Homo sapiens ]
• Synonyms: ADK2; EC 2.7.4.3; AK 2; OTTHUMP00000004287; OTTHUMP00000004288; ATP-AMP transphosphorylase; ATP-AMP transphosphorylase 2; adenylate kinase 2; adenylate kinase isoenzyme 2, mitochondrial; adenylate kinase, mitochondrial
• Gene ID: 204
• mRNA Refseq: NM_001625
• Protein Refseq: NP_001616
• MIM: 103020
• UniProt ID: P54819
• Chromosome Location: 1p34
• Pathway: Purine metabolism; Nucleotide metabolism
• Function: ATP binding ; adenylate kinase activity; nucleoside kinase activity; nucleotide binding ; transferase activity