||Recombinant human BMP-7 protein was expressed in E.coli and purified by using conventional chromatography techniques, after refolding of the isolated inclusion body in renaturation buffer, 16.8 kDa (148 aa).
||Bone morphogenic protein-7 (BMP-7/OP-1) is a member of the transforming growth factor-beta (TGF-beta) superfamily.Like other members of the BMPs family, it plays a role in calcium regulation and bone homeostasis. BMPs have been also shown to regulate the growth, differentiation, chemotaxis and apoptosis of various cell types, including mesenchymal cells, epithelial cells, hematopoietic cells and neuronal cells. BMP7 also has the potential for treatment of chronic kidney disease.
|Sequences of amino acids:
||MSTGSKQRSQ NRSKTPKNQE ALRMANVAEN SSSDQRQACK KHELYVSFRD LGWQDWIIAP EGYAAYYCEG ECAFPLNSYM NATNHAIVQT LVHFINPETV PKPCCAPTQL NAISVLYFDD SSNVILKKYR NMVVRACGCH LEHHHHHH
||Liquid. In 10mM Sodium Citrate buffer (pH 3.5) containing 10% glycerol
||> 95% by SDS - PAGE
||0.5 mg/ml (determined by Bradford assay)
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.