Recombinant Human Complexin 1, His-tagged CPLX1-305H

Recombinant Human Complexin 1, His-tagged

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Recombinant Human Complexin 1, His-tagged

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Cat. No. : CPLX1-305H
Product Overview : Recombinant Complexin 1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (154 aa, 17.1 kDa).
Description : Complexin1 belongs to a family of SNARE complex binding proteins that were suggested to be either facilitators or inhibitors of vesicle exocytosis. Complexin 1 exhibits reduced Ca2+-triggered fast neurotransmitter release at hippocampal glutamatergic synapses, indicating that this protein is a positive regulator of transmitter release. In contrast, it inhibits SNARE-mediated liposome and cell fusions in vitro, which lead to the hypothesis that it acts as fusion clamps of synaptic exocytosis.
Source : E.coli.
Formulation : Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol.
Molecular Weight : 17.1 kDa (154 aa) (Real molecular weight on SDS-PAGE will be shift up).
Purity : > 90% by SDS – PAGE.
Concentration : 1 mg/ml (determined by Bradford assay).
Endotoxin Level : < 1.0 EU per 1 μg of protein (determined by LAL method).
Sequence : MGSSHHHHHH SSGLVPRGSH MEFVMKQALG GATKDMGKML GGDEEKDPDA AKKEEERQEA LRQAEEERKA KYAKMEAERE AVRQGIRDKY GIKKKEEREA EAQAAMEANS EGSLTRPKKA IPPGCGDEVE EEDESILDTV IKYLPGPLQD MLKK
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
PDBrendering based on 1kil.Available structures:1kil. :

Gene Name : CPLX1
Synonyms : CPLX1; complexin 1; CPX1; CPX-I; CPLX1; CPX I; Complexin I; complexin 1 synaphin 2; Synaphin-2.
Gene ID : 10815
mRNA Refseq : NM_006651.3
Protein Refseq : NP_006642.1
MIM : 605032
UniProt ID : O14810
Chromosome Location : 4p16.3
Pathway : Synaptic Transmission.
Function : syntaxin binding.

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