Active Recombinant Human TLR4/LY96 Complex Protein, His-tagged

• Cat.No.: TLR4-296H
• Product Overview: Recombinant Human TLR4(His19-Glu563)/LY96(Glu17 - Asn160) fused with His tag at C-terminal was expressed in NS0.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Glu24 (TLR4) and Glu17 (MD-2)
• Form: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured by its ability to inhibit LPS-induced TNF-alpha secretion by PMA-differentiated U937 human histiocytic lymphoma cells. Typically 20 µg/mL of rhTLR4/MD-2 will inhibit >60% of the TNF-alpha secretion induced by 10 ng/mL of LPS.
• Molecular Mass: Predicted Molecular Mass: 70.6 (TLR4), 19.2 (MD-2) kDa;
  SDS-PAGE: 95 kDa and 34 kDa, reducing conditions.
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Storage: Avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: TLR4 toll-like receptor 4 [ Homo sapiens ]
• Official Symbol: TLR4
• Synonyms: TLR4; toll-like receptor 4; CD284; hToll; TLR 4; homolog of Drosophila toll; TOLL; TLR-4; ARMD10
• Gene ID: 7099
• mRNA Refseq: NM_003266
• Protein Refseq: NP_003257
• UniProt ID: O00206
• Chromosome Location: 9q33.1
• Pathway: Activated TLR4 signalling, organism-specific biosystem; Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon, organism-specific biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Chagas disease (American trypanosomiasis), organism-specific biosystem; Chagas disease (American trypanosomiasis), conserved biosystem; IKK complex recruitment mediated by RIP1, organism-specific biosystem
• Function: lipopolysaccharide binding; lipopolysaccharide binding; lipopolysaccharide receptor activity; phosphatidylinositol 3-kinase binding; protein binding; receptor activity; transmembrane signaling receptor activity