Native Human Osteoprotegerin

• Cat.No.: TNFRSF11B-54H
• Product Overview: The recombinant protein is produced in human embryo kidney cells. The cysteine-rich region of human Osteoprotegerin (aa 22-202) is fused to the Fc portion of human IgG1. The chimeric protein has an apparent molecular mass of 61 kDa by SDS-PAGE (reducing conditions).

Specification

• Source: human embryo kidney
• Species: Human
• Tag: HIgG1, Fc-tagged
• Form: Lyophilized human Osteoprotegerin, 1 mg/ml in PBS after constitution.
• Purity: >95% as determined by SDS-PAGE.
• Applications: Inibition of TRAIL-mediated apoptosis. The rhOPG:Fc recombinant protein exerts its inhibitory activity in the concentration range of 5-20 μg/ml. (Human Jurkat lymphoma cells (50,000 cells in 100 ul DMEM medium containing 5% fetal calf serum) were incubated with 0.1 μg/ml sTRAIL plus 2 ug/ml enhancer (anti-flag antibody M2) and increaing concentrations of rhOPG:Fc fusion protein in a 96-well plate for 16 hr at 37°C. Concentrations of rhOPG:Fc required to inhibit may vary depending on the cell type studied and on the concentration of sTRAIL used to kill the cells.
• Usage: For in vitro research use only. Not for use in diagnostics or in humans.
• Storage: This product is stable at –20°C for at least 6 months. Once rehydrated it is recommended to prepare appropriate aliquots and to store them at –20°C. Avoid repeated freezing and thawing (activity may decrease).
• Warning: No warranties, expressed or implied, are made regarding the use of this product. Creative Biomart is not liable for any damage, personal injury, or economic loss caused by this product.

Gene Information

• Gene Name: TNFRSF11B tumor necrosis factor receptor superfamily, member 11b [ Homo sapiens ]
• Official Symbol: TNFRSF11B
• Synonyms: TNFRSF11B; tumor necrosis factor receptor superfamily, member 11b; OPG, osteoprotegerin; tumor necrosis factor receptor superfamily member 11B; OCIF; TR1; osteoprotegerin; osteoclastogenesis inhibitory factor; OPG; MGC29565
• Gene ID: 4982
• mRNA Refseq: NM_002546
• Protein Refseq: NP_002537
• MIM: 602643
• UniProt ID: O00300
• Chromosome Location: 8q24
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Monoamine Transport, organism-specific biosystem; Osteoblast Signaling, organism-specific biosystem; Osteoclast Signaling, organism-specific biosystem; Osteoclast differentiation, organism-specific biosystem; Osteoclast differentiation, conserved biosystem
• Function: binding; cytokine activity; receptor activity