Human Jurkat clone E6-1 lysate
Cat.No. : | Jurkat-21H |
- Specification
- Gene Information
- Related Products
Species : | Human |
Concentration : | 2 mg/ml |
Tissue Type : | Acute T Cell Leukemia |
Preparation method : | Tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 g/ml of aprotinin, 5 g/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% -mercaptoethanol. |
Recommended Usage : | For research use only, not for diagnostic or therapeutic use. |
Storage Buffer : | Lysate is supplied in SDS sample buffer containing 5% -mercaptoethanol |
Applications : | Protein Lysate for WB. Western blot-Load 5-20 ug/lane for testing with various antibodies. Heat at 95oC for 2-5 minutes prior to loading on gels. |
Products Types
◆ Lysates | ||
Jurkat-039WCY | Human T cell lymphoblast-like cell line Jurkat Whole Cell Lysate | +Inquiry |
Jurkat-166H | Jurkat Whole Cell Lysate | +Inquiry |
Jurkat-256H | Human Jurkat Membrane Lysate | +Inquiry |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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