||Recombinant human FABP1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques, 16kDa (147aa).
||FABP1 (Fatty acid binding protein1) encodes the fatty acid binding protein found in liver. FABP1 is composed of ten antiparallel β strands that form a barrel with a bigger binding pocket than the other FABPs allowing it to accommodate two fatty acid. This protein binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm; it may be involved in intracellular lipid transport and metabolism.
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MSFSGKYQLQ SQENFEAFMK AIGLPEELIQ KGKDIKGVSE IVQNGKHFKF TITAGSKVIQ NEFTVGEECE LETMTGEKVK TVVQLEGDNK LVTTFKNIKS VTELNGDIIT NTMTLGDIVF KRISKRI
||Liquid. In 20 mM Tris-HCl buffer (pH 8.0)
||> 95% by SDS - PAGE
||1 mg/ml (determined by Bradford assay)
||< 1.0 EU per 1ug of protein (determined by LAL method)
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.