Recombinant Human Glyceraldehyde-3-phosphate dehydrogenase

• Cat.No.: GAPDH-115H
• Product Overview: Recombinant GAPDH protein was expressed in E.coli and purified by using conventional chromatography techniques, 36kDa (335aa).

Specification

• Cat. No.: GAPDH-115H
• Description: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a catalytic enzyme commonly known to be involved in glycolysis. The enzyme exists as a tetramer composed of 36-kDa subunits and has various intracellular functions. GAPDH catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Besides functioning as a glycolytic enzyme in cytoplasm, evidence suggests that mammalian GAPDH is also involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication and DNA repair.
• Sequences of amino acids: MGKVKVGVNG FGRIGRLVTR AAFNSGKVDI VAINDPFIDL NYMVYMFQYD STHGKFHGTV KAENGKLVIN GNPITIFQER DPSKIKWGDA GAEYVVESTG VFTTMEKAGA HLQGGAKRVI ISAPSADAPM FVMGVNHEKY DNSLKIISNA SCTTNCLAPL AKVIHDNFGI VEGLMTTVHA ITATQKTVDG PSGKLWRDGR GALQNIIPAS TGAAKAVGKV IPELNGKLTG MAFRVPTANV SVVDLTCRLE KPAKYDDIKK VVKQASEGPL KGILGYTEHQ VVSSDFNSDT HSSTFDAGAG IALNDHFVKL ISWYDNEFGY SNRVVDLMAH MASKE
• Formulation: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 1 mM EDTA, 20% glycerol
• Purity: > 95% by SDS - PAGE
• Concentration: 1 mg/ml (determined by Bradford assay)
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Publication:
  Myeloperoxidase catalyzes the conjugation of serotonin to thiols via free radicals and tryptamine-4, 5-dione (2012)

Gene Information

• Gene Name: GAPDH glyceraldehyde-3-phosphate dehydrogenase [ Homo sapiens ]
• Synonyms: glyceraldehyde-3-phosphate dehydrogenase; G3PD; GAPD; MGC88685; GAPDH;glyceraldehyde-3-phosphate dehydrogenase; OTTHUMP00000174431; EC 1.2.1.12; OTTHUMP00000174432; aging-associated gene 9 protein; glyceraldehyde 3-phosphate dehydrogenase
• Gene ID: 2597
• mRNA Refseq: NM_002046
• Protein Refseq: NP_002037
• MIM: 138400
• UniProt ID: P04406
• Chromosome Location: 12p13
• Pathway: Alzheimer"s disease; Dentatorubropallidoluysian atrophy (DRPLA); Glycolysis / Gluconeogenesis; Huntington"s disease; Neurodegenerative Disorders; Metabolism of carbohydrates
• Function: NAD or NADH binding; glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity; oxidoreductase activity; protein binding