||Recombinant humanLatexin protein was expressed in E.coli and purified by using conventional chromatography, 25.7 kDa (222aa).
||Latexin, a carboxypeptidase A inhibitor, is highly expressed in heart, prostate, ovary, kidney, pancrease, and colon, moderaqte or low in other tissues including brain. Latexin has no detectable sequence similarity with plant and parasite inhibitors, but it is related to a human putative tumor suppressor protein, TIG1. It is down-regulated in the presenilin-1-deficient mouse brain, thus putatively playing a role in Alzheimer"s disease.
|Sequences of amino acids:
||MEIPPTNYPA SRAALVAQNY INYQQGTPHR VFEVQKVKQA SMEDIPGRGH KYRLKFAVEE IIQKQVKVNC TAEVLYPSTG QETAPEVNFT FEGETGKNPD EEDNTFYQRL KSMKEPLEAQ NIPDNFGNVS PEMTLVLHLA WVACGYIIWQ NSTEDTWYKM VKIQTVKQVQ RNDDFIELDY TILLHNIASQ EIIPWQMQVL WHPQYGTKVK HNSRLPKEVQ LE
||Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing 50 mM NaCl, 10% glycerol
||> 95% by SDS - PAGE
||1 mg/ml (determined by Bradford assay)
||< 1.0 EU per 1 μg of protein (determined by LAL method )
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.