||Recombinant human RheB protein, fused to T7-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques, 21.7 kDa (197 aa).
||Ras homolog enriched in brain (RheB) is a member of the Ras superfamily that was originally identified as an immediate-early gene in brain but is also widely expressed in other tissues. RheB encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. RheB is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway.
||Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT 10% glycerol.
||> 95% by SDS - PAGE.
||1 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MASMTGGQQM GRGSASMPQS KSRKIAILGY RSVGKSSLTI QFVEGQFVDS YDPTIENTFT KLITVNGQEY HLQLVDTAGQ DEYSIFPQTY SIDINGYIL V YSVTSIKSFE VIKVIHGKLL DMVGKVQIPI MLVGNKKDLH MERVISYEEG KALAESWNAA FLESSAKENQ TAVDVFRRII LEAEKMDGAA SQGKSSC
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
||Insulin signaling pathway; mTOR signaling pathway; Signaling by Insulin receptor