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Recombinant Human Ras Homolog Enriched in Brain, T7-Tagged

Cat. No.: RHEB-393H
Product Overview: Recombinant human RheB protein, fused to T7-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques, 21.7 kDa (197 aa).
Description: Ras homolog enriched in brain (RheB) is a member of the Ras superfamily that was originally identified as an immediate-early gene in brain but is also widely expressed in other tissues. RheB encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. RheB is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway.
Source: Escherichia Coli.
Form: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT 10% glycerol.
Purity: > 95% by SDS - PAGE.
Concentration: 1 mg/ml (determined by Bradford assay)
Sequences of amino acids: MASMTGGQQM GRGSASMPQS KSRKIAILGY RSVGKSSLTI QFVEGQFVDS YDPTIENTFT KLITVNGQEY HLQLVDTAGQ DEYSIFPQTY SIDINGYIL V YSVTSIKSFE VIKVIHGKLL DMVGKVQIPI MLVGNKKDLH MERVISYEEG KALAESWNAA FLESSAKENQ TAVDVFRRII LEAEKMDGAA SQGKSSC
Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Pathways: Insulin signaling pathway; mTOR signaling pathway; Signaling by Insulin receptor
Gene Name: RHEB Ras homolog enriched in brain [ Homo sapiens ]
Synonyms: Ras homolog enriched in brain; RHEB2; MGC111559; RHEB; GTP-binding protein Rheb; Ras homolog enriched in brain 2; Ras homolog enriched in brain.
Gene ID: 6009
mRNA Refseq: NM_005614
Protein Refseq: NP_005605
MIM: 601293
UniProt ID: Q15382
Chromosome Location: 7q36
Function: GTP binding; GTPase activity; nucleotide binding.

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