Recombinant Human APP/Protease Nexin II, CF APP-54H

Recombinant Human APP/Protease Nexin II, CF

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Recombinant Human APP/Protease Nexin II, CF

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Cat.No. : APP-54H
Product Overview : A DNA sequence encoding the human CD33 signal peptide and the ectodomain of the recombinant human A Disintegrin and Metalloprotease domain 10 (rhADAM10, amino residues 18 - 672) (Rosendahl, et al., 1997, J. Biol. Chem. 272:24588 - 24593) was expressed with a C-terminal 10X His tag in an insect cell line, Sf21.
Description : ADAM10 (also known as Kuzbanian, mammalian disintegrin metalloprotease, myelin-associated metalloproteinase) is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain. Like other membrane-anchored ADAMs, ADAM10 consists of the following domains, pro with a cysteine switch and furin cleavage sequence, catalytic with the zinc-binding site and Met-turn expected for reprolysins, disintegrin-like, cystein-rich, EGF-like, transmembrane, and cytoplasmic. ADAM10 is highly conserved, with 97% amino acid identity between mouse, rat, cattle and human and 45% identity between mouse and the active enzyme processes notch, notch ligand delta, and amyloid protein precursor at the alpha site, playing an important role in neurogenesis.
Source : Insect Sf21 cell line
Species : Human
Tag : His
Bio-activity : Measured by its ability to cleave a fluorescent peptide substrate, (7-methoxycoumarin-4-yl) acetyl-Pro-Leu-Ala-Gln-Ala-Val-(3-[2, 4-dinitrophenyl]-L-2, 3-diaminopropionyl)-Arg-Ser-Ser-Ser-Arg -NH2 . Cleavage of ES003 can be measured using excitation and emission wavelengths of 320 nm and 405 nm, respectively.
Molecular Mass : The purified secreted rmADAM10 exists as the mature and active form with the N-terminal sequence of T214TSAEKNTCQ. The 469 amino acid residue rhADAM10 has a predicted molecular mass of approximately 52 kDa. By SDS-PAGE, the apparent molecular mass is appr
Endotoxin : < 2.0 eu per 1 μg of the enzyme as determined by the lal
Purity : >90%, as determined by SDS-PAGE and visualized by silver stain
Storage : Lyophilized samples are stable for up to six months at -20° C to -70° C. Upon reconstitution, this cytokine, in the presence of a carrier protein, can be stored under sterile conditions at 2 - 8° C for one month or at -20° C to -70° C in a manual defrost freezer for three months without detectable loss of activity.
Gene Name : APP amyloid beta (A4) precursor protein [ Homo sapiens ]
Official Symbol : APP
Synonyms : APP; amyloid beta (A4) precursor protein; AD1, Alzheimer disease; amyloid beta A4 protein; peptidase nexin II; preA4; protease nexin-II; peptidase nexin-II; beta-amyloid peptide; alzheimer disease amyloid protein; cerebral vascular amyloid peptide; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma;
Gene ID : 351
mRNA Refseq : NM_000484
Protein Refseq : NP_000475
MIM : 104760
UniProt ID : P05067
Chromosome Location : 21q21.2
Pathway : Activated TLR4 signalling, organism-specific biosystem; Advanced glycosylation endproduct receptor signaling, organism-specific biosystem; Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Amyloids, organism-specific biosystem; Caspase cascade in apoptosis, organism-specific biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem;
Function : DNA binding; PTB domain binding; acetylcholine receptor binding; heparin binding; identical protein binding; peptidase activator activity; peptidase inhibitor activity; protein binding; receptor binding; serine-type endopeptidase inhibitor activity; transition metal ion binding;

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