Active Recombinant Human APP/Protease Nexin II, CF

• Cat.No.: APP-54H
• Product Overview: A DNA sequence encoding the human CD33 signal peptide and the ectodomain of the recombinant human A Disintegrin and Metalloprotease domain 10 (rhADAM10, amino residues 18 - 672) (Rosendahl, et al., 1997, J. Biol. Chem. 272:24588 - 24593) was expressed with a C-terminal 10X His tag in an insect cell line, Sf21.

Specification

• Description: ADAM10 (also known as Kuzbanian, mammalian disintegrin metalloprotease, myelin-associated metalloproteinase) is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain. Like other membrane-anchored ADAMs, ADAM10 consists of the following domains, pro with a cysteine switch and furin cleavage sequence, catalytic with the zinc-binding site and Met-turn expected for reprolysins, disintegrin-like, cystein-rich, EGF-like, transmembrane, and cytoplasmic. ADAM10 is highly conserved, with 97% amino acid identity between mouse, rat, cattle and human and 45% identity between mouse and the active enzyme processes notch, notch ligand delta, and amyloid protein precursor at the alpha site, playing an important role in neurogenesis.
• Source: Insect Sf21 cell line
• Species: Human
• Tag: His
• Bio-activity: Measured by its ability to cleave a fluorescent peptide substrate, (7-methoxycoumarin-4-yl) acetyl-Pro-Leu-Ala-Gln-Ala-Val-(3-[2, 4-dinitrophenyl]-L-2, 3-diaminopropionyl)-Arg-Ser-Ser-Ser-Arg -NH2 . Cleavage of ES003 can be measured using excitation and emission wavelengths of 320 nm and 405 nm, respectively.
• Molecular Mass: The purified secreted rmADAM10 exists as the mature and active form with the N-terminal sequence of T214TSAEKNTCQ. The 469 amino acid residue rhADAM10 has a predicted molecular mass of approximately 52 kDa. By SDS-PAGE, the apparent molecular mass is appr
• Endotoxin: < 2.0 eu per 1 μg of the enzyme as determined by the lal
• Purity: >90%, as determined by SDS-PAGE and visualized by silver stain
• Storage: Lyophilized samples are stable for up to six months at -20° C to -70° C. Upon reconstitution, this cytokine, in the presence of a carrier protein, can be stored under sterile conditions at 2 - 8° C for one month or at -20° C to -70° C in a manual defrost freezer for three months without detectable loss of activity.

Gene Information

• Gene Name: APP amyloid beta (A4) precursor protein [ Homo sapiens ]
• Official Symbol: APP
• Synonyms: APP; amyloid beta (A4) precursor protein; AD1, Alzheimer disease; amyloid beta A4 protein; peptidase nexin II; preA4; protease nexin-II; peptidase nexin-II; beta-amyloid peptide; alzheimer disease amyloid protein; cerebral vascular amyloid peptide; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma
• Gene ID: 351
• mRNA Refseq: NM_000484
• Protein Refseq: NP_000475
• MIM: 104760
• UniProt ID: P05067
• Chromosome Location: 21q21.2
• Pathway: Activated TLR4 signalling, organism-specific biosystem; Advanced glycosylation endproduct receptor signaling, organism-specific biosystem; Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Amyloids, organism-specific biosystem; Caspase cascade in apoptosis, organism-specific biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem
• Function: DNA binding; PTB domain binding; acetylcholine receptor binding; heparin binding; identical protein binding; peptidase activator activity; peptidase inhibitor activity; protein binding; receptor binding; serine-type endopeptidase inhibitor activity; transition metal ion binding