Recombinant Human LTA4H Protein, His-tagged

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Recombinant Human LTA4H Protein, His-tagged

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Cat.No. : LTA4H-51H
Product Overview : Recombinant human LTA4H(1-610) protein with a C-terminal His-tag was expressed in E. coli.
Description : The protein encoded by this gene is an enzyme that contains both hydrolase and aminopeptidase activities. The hydrolase activity is used in the final step of the biosynthesis of leukotriene B4, a proinflammatory mediator. The aminopeptidase activity has been shown to degrade proline-glycine-proline (PGP), a neutrophil chemoattractant and biomarker for chronic obstructive pulmonary disease (COPD). Several transcript variants encoding different isoforms have been found for this gene.
Source : E. coli
Species : Human
Tag : His
Molecular Mass : 69 kDa
Protein length : 1-610
Purity : ≥90% estimated by SDS-PAGE
Unit Definition : One unit is the amount of enzyme required to release 1 mmol per min of 4-nitro-anilide (pNA) at 26 centigrade in a reaction containing 40 mM L-Ala-pNA in 50 mM Tris pH 8.0, measured by absorbance at 405 nm.
Applications : Enzyme Activity
Stability : ≥ 1 year
Storage : Store it at -80 centigrade
Storage Buffer : 100 mM Tris, pH 8.0, with 100 mM potassium chloride and 20% glycerol
SDS-PAGE Analysis of LTA4H :

Lane 1: MW Markers; Lane 2: LTA4 Hydrolase (2 μg); Lane 3: LTA4 Hydrolase (4 μg). Representative gel image shown; actual purity may vary between each batch.

LTA4 Hydrolase Activity :

Gene Name : LTA4H leukotriene A4 hydrolase [ Homo sapiens (human) ]
Official Symbol : LTA4H
Synonyms : LTA4H; leukotriene A4 hydrolase; leukotriene A-4 hydrolase; LTA-4 hydrolase; testicular secretory protein Li 27
Gene ID : 4048
mRNA Refseq : NM_000895
Protein Refseq : NP_000886
MIM : 151570
UniProt ID : P09960

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