Recombinant Human F7 therapeutic protein

• Cat.No.: F7-P042H
• Product Overview: Recombinant human coagulation Factor VIIa (rFVIIa), intended for promoting hemostasis by activating the extrinsic pathway of the coagulation cascade. It is a vitamin K-dependent glycoprotein consisting of 406 amino acid residues. Cloned and expressed in hamster kidney cells, the protein is catalytically active in a two-chain form.

Specification

• Description: This gene encodes coagulation factor VII which is a vitamin K-dependent factor essential for hemostasis. This factor circulates in the blood in a zymogen form, and is converted to an active form by either factor IXa, factor Xa, factor XIIa, or thrombin by minor proteolysis. Upon activation of the factor VII, a heavy chain containing a catalytic domain and a light chain containing 2 EGF-like domains are generated, and two chains are held together by a disulfide bond. In the presence of factor III and calcium ions, the activated factor then further activates the coagulation cascade by converting factor IX to factor IXa and/or factor X to factor Xa. Alternative splicing of this gene results in 2 transcripts. Defects in this gene can cause coagulopathy. The expression product is the active ingredient of Novoseven and Niastase.
• Source: Hamster kidney cells
• Species: Human
• Molecular Mass: 45.1 kDa
• Protein length: 406 aa
• AA Sequence: ANAFLEELRPGSLERECKEEQCSFE EAREIFKDAERTKLFWISYSDGDQC ASSPCQNGGSCKDQLQSYICF CLPAFEGRNCETHKDDQLICVNENG GCEQYCSDHTGTKRSCRCHEGYSLL ADGVSCTPTVEYPCGKIPILE KRNASKPQGRIVGGKVCPKGECPWQ VLLLVNGAQLCGGTLINTIWVVSAA HCFDKIKNWRNLIAVLGEHDL SEHDGDEQSRRVAQVIIPSTYVPGT TNHDIALLRLHQPVVLTDHVVPLCL PERTFSERTLAFVRFSLVSGW GQLLDRGATALELMVLNVPRLMTQD CLQQSRKVGDSPNITEYMFCAGYSD GSKDSCKGDSGGPHATHYRGT WYLTGIVSWGQGCATVGHFGVYTRV SQYIEWLQKLMRSEPRPGVLLRAPF P
• Endotoxin: < 0.1 EU per μg of the protein
• Purity: >95%
• Storage: Can be stored at +4 centigrade short term (1-2weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoidrepeated freezing and thawing cycles.

Gene Information

• Gene Name: F7 coagulation factor VII (serum prothrombin conversion accelerator) [ Homo sapiens ]
• Official Symbol: F7
• Synonyms: F7; coagulation factor VII (serum prothrombin conversion accelerator); coagulation factor VII; eptacog alfa; factor VII; FVII coagulation protein; SPCA; proconvertin; serum prothrombin conversion accelerator
• Gene ID: 2155
• mRNA Refseq: NM_000131
• Protein Refseq: NP_000122
• MIM: 613878
• UniProt ID: P08709
• Chromosome Location: 13q34
• Pathway: BMAL1:CLOCK/NPAS2 Activates Circadian Expression, organism-specific biosystem; Blood Clotting Cascade, organism-specific biosystem; Circadian Clock, organism-specific biosystem; Complement and Coagulation Cascades, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Extrinsic Pathway, organism-specific biosystem
• Function: calcium ion binding; glycoprotein binding; peptidase activity; receptor binding; serine-type endopeptidase activity; serine-type peptidase activity