Recombinant Human PRKCZ, His-tagged

• Cat.No.: PRKCZ-4811H
• Product Overview: Recombinant Human Protein Kinase C Zeta type/PRKCZ is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Met1-Val592) of Human PRKCZ fused with a polyhistidine tag at the C-terminus.

Specification

• Description: Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Source: HEK293
• Species: Human
• Tag: His
• AA Sequence: MPSRTGPKMEGSGGRVRLKAHYGGD IFITSVDAATTFEELCEEVRDMCRL HQQHPLTLKW VDSE GDPCTVSSQMELEEAFRLARQCRDE GLIIHVFPSTPEQPGLPCPGEDKSI YRRGAR RWRKLYRA NGHLFQAKRFNRRAYCGQCSERIWG LARQGYRCINCKLLVHKRCHGLVPL TC RKHMDSVMPSQE PPVDDKNEDADLPSEETDGIAYISS SRKHDSIKDDSEDLKPVIDGMDGIK ISQGLGLQDFDLIRV IGRGSYAKVLLVRLKKNDQIYAMKV VKKELVHDDEDIDWVQTE KHVFEQASSNPFLVGLHSCFQ TTSRLFLVIEYVNGGDLMFHMQRQR KLPEEHARFYAAEI CIALNFLHERGIIYRDLKLDNVL LDADGHIKLTDYGMCKEGLGPGDTT STFCGTPNYIAP EILRGEEYGFSVDWWALGVLMFEMM AG RSPFDIITDNPDMNTEDYLFQVILE KPIRIPRF LSVKASHVLKGFLNKDPKERLGCRP QTGFSD IKSHAFFRSIDWDLLEKKQALPPFQ PQIT DDYGLDNFDTQFTSEPVQLTPDDED AIKRIDQSE FEGFEYINPLLLSTEESV
• Endotoxin: Less than 0.1 ng/μg (1 IEU/μg).
• Purity: Greater than 95% as determined by reducing SDS-PAGE.

Gene Information

• Gene Name: PRKCZ protein kinase C, zeta [ Homo sapiens (human) ]
• Official Symbol: PRKCZ
• Synonyms: PRKCZ; protein kinase C, zeta; protein kinase C zeta type; PKC2; nPKC-zeta; PKC-ZETA; NP_001028753.1; EC 2.7.11.13; NP_001028754.1; NP_001229803.1; NP_002735.3
• Gene ID: 5590
• mRNA Refseq: NM_001033581
• Protein Refseq: NP_001028753
• MIM: 176982
• UniProt ID: Q05513
• Chromosome Location: 1p36.33-p36.2
• Pathway: AGE/RAGE pathway; CDC42 signaling events; CXCR4-mediated signaling events
• Function: 14-3-3 protein binding; ATP binding; insulin receptor substrate binding; metal ion binding