||Recombinant Human TDO2, fused with C-terminal MYC/DDK, was expressed in HEK293 cells.
||This gene encodes a heme enzyme that plays a critical role in tryptophan metabolism by catalyzing the first and rate-limiting step of the kynurenine pathway. Increased activity of the encoded protein and subsequent kynurenine production may also play a role in cancer through the suppression of antitumor immune responses, and single nucleotide polymorphisms in this gene may be associated with autism.
||The specific activity of TDO2 was determined by monitoring Kynurenine formation from the N-formylkynurenine based on the absorbance at 492nm.
||> 80% as determined by SDS-PAGE and Coomassie blue staining
||>50 ug/mL as determined by microplate BCA method