Recombinant Human MFGE8, His-tagged MFGE8-408H

Recombinant Human MFGE8, His-tagged

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Recombinant Human MFGE8, His-tagged

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Cat.No. : MFGE8-408H
Product Overview : Recombinant Human MFGE8(Leu24-Cys387), fused with a C-terminal 6-His tag, was expressed in Mouse NS0 Cells.
Description : Milk fat globule-EGF factor 8 protein (Mfge8), also known as lactadherin, is a protein which in humans is encoded by the MFGE8 gene.
Source : Mouse NS0 Cells
Species : Human
Tag : His
Predicted N Terminal : Leu24
Form : Lyophilized from a 0.2 µm filtered solution in NaH2PO4 and NaCl.
Bio-activity : Measured by the ability of the immobilized protein to support the adhesion of SVEC4-10 mouse vascular endothelial cells. When 5 x 10^4 cells/well are added to Recombinant Human MFG-E8 coated plates (10 µg/mL, 100 µL/well), approximately 45-75% will adhere after 60 minutes at 37 ºC.
Molecular Mass : Predicted Molecular Mass: 41.6 kDaSDS-PAGE: 45-50 kDa, reducing conditions
Endotoxin : < 1.0 EU per 1 μg of the protein by the LAL method.
Purity : >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 ºC as supplied.1 month, 2 to 8 ºC under sterile conditions after reconstitution.3 months, -20 to -70 ºC under sterile conditions after reconstitution.
Reconstitution : Reconstitute at 100 µg/mL in sterile PBS.
Gene Name : MFGE8 milk fat globule-EGF factor 8 protein [ Homo sapiens (human) ]
Official Symbol : MFGE8
Synonyms : MFGE8; milk fat globule-EGF factor 8 protein; BA46; HMFG; MFGM; SED1; hP47; EDIL1; MFG-E8; SPAG10; OAcGD3S; HsT19888; lactadherin; O-acetyl disialoganglioside synthase; breast epithelial antigen BA46; medin; sperm associated antigen 10; sperm surface protein hP47
Gene ID : 4240
mRNA Refseq : NM_001114614
Protein Refseq : NP_001108086
MIM : 602281
UniProt ID : Q08431
Chromosome Location : 15q25
Pathway : Amyloids, organism-specific biosystem; Disease, organism-specific biosystem; Integrins in angiogenesis, organism-specific biosystem
Function : integrin binding; phosphatidylethanolamine binding; phosphatidylserine binding

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