Active Recombinant Human MFGE8, His-tagged

• Cat.No.: MFGE8-408H
• Product Overview: Recombinant Human MFGE8(Leu24-Cys387), fused with a C-terminal 6-His tag, was expressed in Mouse NS0 Cells.

Specification

• Description: Milk fat globule-EGF factor 8 protein (Mfge8), also known as lactadherin, is a protein which in humans is encoded by the MFGE8 gene.
• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Leu24
• Form: Lyophilized from a 0.2 µm filtered solution in NaH2PO4 and NaCl.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of SVEC4-10 mouse vascular endothelial cells. When 5 x 10^4 cells/well are added to Recombinant Human MFG-E8 coated plates (10 µg/mL, 100 µL/well), approximately 45-75% will adhere after 60 minutes at 37 ºC.
• Molecular Mass: Predicted Molecular Mass: 41.6 kDaSDS-PAGE: 45-50 kDa, reducing conditions
• Endotoxin: < 1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 ºC as supplied.1 month, 2 to 8 ºC under sterile conditions after reconstitution.3 months, -20 to -70 ºC under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS.

Gene Information

• Gene Name: MFGE8 milk fat globule-EGF factor 8 protein [ Homo sapiens (human) ]
• Official Symbol: MFGE8
• Synonyms: MFGE8; milk fat globule-EGF factor 8 protein; BA46; HMFG; MFGM; SED1; hP47; EDIL1; MFG-E8; SPAG10; OAcGD3S; HsT19888; lactadherin; O-acetyl disialoganglioside synthase; breast epithelial antigen BA46; medin; sperm associated antigen 10; sperm surface protein hP47
• Gene ID: 4240
• mRNA Refseq: NM_001114614
• Protein Refseq: NP_001108086
• MIM: 602281
• UniProt ID: Q08431
• Chromosome Location: 15q25
• Pathway: Amyloids, organism-specific biosystem; Disease, organism-specific biosystem; Integrins in angiogenesis, organism-specific biosystem
• Function: integrin binding; phosphatidylethanolamine binding; phosphatidylserine binding