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Recombinant E. coli RuvC

Cat.No. : ruvC-133E
Product Overview : Full-length recombinant protein expressed in E.coli and highly purified by combined chromatography
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  • Gene Information
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Description : E. coli RuvC protein (19 kDa) is a structurally specific endonuclease which binds specifically to the Holliday structure, an intermediate of recombination, at the late stage of homologous recombination and recombination repair and introduces a nick in the symmetrical point of the Holliday junction cleaving and resolving the recombinant. Functional form is dimmer.
Source : E. coli
Species : E. coli
Form : 50% glycerol, 10 mM Tris-HCl (pH7.5), 2 mM EDTA, 100 mM NaCl, 5 mM mercaptoethanol
Purity : Over 90% by SDS-PAGE (CBB staining)
Applications : 1) Functional studies in vitro. RuvC cleaves recombination intermediate at Holliday Junction. 2) SDS-PAGE3) Standard antigen for western blotting and ELISA
Storage : Sent at 4℃or at -20℃ and upon arrival, centrifuge briefly and store at -20℃ or -80℃ for longer period.
Concentration : 1.0 mg/ml (determined by BCA method)

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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