Active Recombinant Human CDH11, Fc Chimera

• Cat.No.: CDH11-159H
• Product Overview: Recombinant Human CDH11 (Accession # AAA35622) was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: Fc Chimera
• Predicted N Terminal: Phe23 (pro) & Gly54 (mature)
• Form: Lyophilized from a 0.2 µm filtered solution in MES and NaCl with Trehalose.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of MDA-MB-231 human breast cancer cells.When 7.5 x 10^4 cells/well are added to Recombinant Human Cadherin-11 Fc Chimera coated plates (20 µg/mL with 100 µL/well), approximately 50-80% will adhere after 1 hour at 37 °C.
• Molecular Mass: Recombinant Human CDH11, Fc Chimera has a calculated MW of 93 kDa (pro), 90 kDa (mature). In SDS-PAGE migrates as 110-120 kDa, reducing conditions.
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: CDH11 cadherin 11, type 2, OB-cadherin (osteoblast) [ Homo sapiens ]
• Official Symbol: CDH11
• Synonyms: CDH11; cadherin 11, type 2, OB-cadherin (osteoblast); cadherin-11; CAD11; OB; OB Cadherin; CDHOB; OSF-4
• Gene ID: 1009
• mRNA Refseq: NM_001797
• Protein Refseq: NP_001788
• MIM: 600023
• UniProt ID: P55287
• Chromosome Location: 16q21