Active Recombinant Mouse Vegfa

• Cat.No.: Vegfa-172M
• Product Overview: Recombinant Mouse Vegfa (Accession # AAA40547) Ala27-Arg190 was produced in Spodoptera frugiperda, Sf 21 (baculovirus)-derived.

Specification

• Source: Baculovirus Sf 21 Insect cells
• Species: Mouse
• Predicted N Terminal: Ala27
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323.The ED50 for this effect is typically 0.8-4 ng/mL.
• Molecular Mass: Recombinant Mouse Vegfa has a calculated MW of 19.4 kDa (monomer). In SDS-PAGE migrates as 42 kDa, non-reducing conditions.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 50 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: Vegfa vascular endothelial growth factor A [ Mus musculus ]
• Official Symbol: Vegfa
• Synonyms: VEGFA; vascular endothelial growth factor A; vascular permeability factor; Vpf; Vegf; Vegf120; Vegf164; Vegf188
• Gene ID: 22339
• mRNA Refseq: NM_001025250
• Protein Refseq: NP_001020421