Active Recombinant Human IGFBP4, Met-tagged

• Cat.No.: IGFBP4-191H
• Product Overview: Recombinant Human IGFBP4 (Accession # AAA62670) Asp22-Glu258, fused with an N-terminal Met, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Predicted N Terminal: Met
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its ability to inhibit the biological activity of IGF-I or IGF-II on MCF-7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083.The ED50 for this effect is typically 0.03-0.09 µg/mL in the presence of 14 ng/mL recombinant human IGF-II.
• Molecular Mass: Recombinant Human IGFBP4, Met-tagged has a calculated MW of 26 kDa. In SDS-PAGE migrates as 32 kDa, reducing conditions.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: IGFBP4 insulin-like growth factor binding protein 4 [ Homo sapiens ]
• Official Symbol: IGFBP4
• Synonyms: IGFBP4; insulin-like growth factor binding protein 4; insulin like growth factor binding protein 4; insulin-like growth factor-binding protein 4; BP 4; HT29 IGFBP; IBP4; IGF binding protein 4; IGFBP 4; IBP-4; IGF-binding protein 4; BP-4; IGFBP-4; HT29-IGFBP
• Gene ID: 3487
• mRNA Refseq: NM_001552
• Protein Refseq: NP_001543
• MIM: 146733
• UniProt ID: P22692
• Chromosome Location: 17q12-q21.1

Reviews

02/25/2019

Accurate protein structural analysis, crucial for our investigations.

10/23/2018

Exceptional protein expression optimization support, greatly appreciated.

06/06/2018

Reliable mass spectrometry analysis, pivotal for proteomics studies.

Q&As

What role does IGFBP4 play in bone growth and development, particularly in regulating osteoblast and osteoclast activity?

IGFBP4 plays a key role in bone development, regulating bone-forming and bone-resorbing cells.

What is the impact of IGFBP4 on cardiovascular health, particularly in atherosclerosis and heart disease?

IGFBP4 impacts cardiovascular health, influencing atherosclerosis and heart disease processes.

What are the effects of IGFBP4 dysregulation on aging and age-related diseases?

Dysregulation of IGFBP4 is linked to aging and the progression of age-related diseases.

How does IGFBP4 contribute to metabolic regulation and insulin sensitivity?

IGFBP4 is involved in metabolic regulation, affecting insulin sensitivity and glucose metabolism.

How does IGFBP4 modulate the activity of insulin-like growth factors (IGFs) in various tissues?

IGFBP4 modulates IGF activity, affecting growth and metabolic functions in tissues.

How do alterations in IGFBP4 expression or function affect wound healing and tissue repair?

Changes in IGFBP4 expression influence wound healing and tissue repair mechanisms.

How does IGFBP4 influence cancer progression, especially in hormone-dependent cancers?

IGFBP4 influences cancer progression, particularly in cancers sensitive to hormonal changes.