Active Recombinant Human SEMA5A, Fc Chimera

• Cat.No.: SEMA5A-234H
• Product Overview: Recombinant Human SEMA5A (Accession # AAC09473) was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: Fc Chimera
• Predicted N Terminal: Glu23
• Form: Lyophilized from a 0.2 µm filtered solution in PBS, EDTA and Tween® 20.
• Bio-activity: Measured by its ability to inhibit proliferation of the A549 human lung carcinoma cells.The ED50 for this effect is typically 100-400 ng/mL.
• Molecular Mass: Recombinant Human SEMA5A, Fc Chimera has a calculated MW of 110 kDa (monomer). In SDS-PAGE migrates as 133 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: SEMA5A sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A [ Homo sapiens ]
• Official Symbol: SEMA5A
• Synonyms: SEMA5A; sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A; SEMAF; semaphorin-5A; semF; sema F; semaphorin F; semaphorin-F; FLJ12815
• Gene ID: 9037
• mRNA Refseq: NM_003966
• Protein Refseq: NP_003957
• MIM: 609297
• UniProt ID: Q13591
• Chromosome Location: 5p15.2