Active Recombinant Mouse Wnt2b

• Cat.No.: Wnt2b-239M
• Product Overview: Recombinant Mouse Wnt2b (Accession # AAC25397) Ser57-Thr389 was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO cells
• Species: Mouse
• Predicted N Terminal: Ser57 & Asp71
• Form: Lyophilized from a 0.2 µm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein.
• Bio-activity: Measured by its ability to inhibit cell proliferation/survival assay using C3H10T1/2 mouse embryonic fibroblast cells.The ED50 for this effect is typically 1-5 µg/ml.
• Molecular Mass: Recombinant Mouse Wnt2b has a calculated MW of 38 kDa. In SDS-PAGE migrates as 41-60 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE with silver staining, under reducing conditions.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: Wnt2b wingless related MMTV integration site 2b [ Mus musculus ]
• Official Symbol: Wnt2b
• Synonyms: WNT2B; wingless related MMTV integration site 2b; protein Wnt-2b; wnt-13; Wnt13
• Gene ID: 22414
• mRNA Refseq: NM_009520
• Protein Refseq: NP_033546