|Product Overview:||Recombinant human SELPLG protein (Gln42-Val295) fused with Fc region of human IgG1 (Pro100-Lys330) at C-terminus was expressed in Chinese Hamster Ovary cell line.|
|Description:||This gene encodes a glycoprotein that functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Alternate splicing results in multiple transcript variants.|
|Bio-activity:||Measured by the ability of the immobilized protein to support the adhesion of CHO Chinese hamster ovary cells transfected with P-Selectin. When 5 x 10^4 cells/well are added to rhPSGL-1 coated plates (3 μg/mL with 100 μL/well), >70% will adhere after 30 minutes at 37 centigrade.
Optimal concentration depends on cell type as well as the application or research objectives^
|Molecular Mass:||52.6 kDa|
|N-terminal Sequence Analysis:||Gln42|
|Endotoxin:||<0.10 EU per 1 μg of the protein by the LAL method.|
|Purity:||>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.|
|Storage:||Avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Storage Buffer:||Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.|
|Preservative:||Reconstitute at 100 μg/mL in sterile PBS.|
|Gene Name:||SELPLG selectin P ligand [ Homo sapiens (human) ]|
|Synonyms:||CD162 antigen; CD162; P-selectin glycoprotein ligand 1; PSGL1; PSGL-1; PSGL-1cutaneous lymphocyte-associated associated antigen; selectin P ligandCLA; SELPLG; CLA|