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Recombinant Human SELPLG, Fc Chimera

Cat.No.: SELPLG-242H
Product Overview: Recombinant Human SELPLG (Accession # AAC50061) was produced in Chinese Hamster Ovary cell line, CHO-derived.
Source: CHO cells
Species: Human
Tag: Fc Chimera
Predicted N Terminal: Thr44
Form: Lyophilized from a 0.2 µm filtered solution in Tris and NaCl.
Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of CHO Chinese hamster ovary cells transfected with P-Selectin.When 5 x 10^4 cells/well are added to rhPSGL-1 coated plates (3 µg/mL with 100 µL/well), approximately 70-85% will adhere after 30 minutes at 37 °C. Optimal concentration depends on cell type as well as the application or research objectives.
Molecular Mass: Recombinant Human SELPLG, Fc Chimera has a calculated MW of 52.6 kDa (monomer). In SDS-PAGE migrates as 100-150 kDa, reducing conditions.
Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name: SELPLG selectin P ligand [ Homo sapiens ]
Official Symbol: SELPLG
Synonyms: SELPLG; selectin P ligand; P-selectin glycoprotein ligand 1; CD162; PSGL 1; cutaneous lymphocyte-associated associated antigen; CLA; PSGL1; PSGL-1;
Gene ID: 6404
mRNA Refseq: NM_001206609
Protein Refseq: NP_001193538
MIM: 600738
UniProt ID: Q14242
Chromosome Location: 12q24

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