Active Recombinant Mouse Serpina1a, His-tagged

• Cat.No.: Serpina1a-304M
• Product Overview: Recombinant Mouse Serpina1a (Accession # AAH11040) Glu25-Lys413, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Glu25
• Form: Supplied as a 0.2 µm filtered solution in Tris and NaCl.
• Bio-activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 .The IC50 is <5 nM, as measured under the described conditions.
• Molecular Mass: Recombinant Mouse Serpina1a, His-tagged has a calculated MW of 44 kDa. In SDS-PAGE migrates as 50-60 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Serpina1a serine (or cysteine) peptidase inhibitor, clade A, member 1A [ Mus musculus (house mouse) ]
• Official Symbol: Serpina1a
• Synonyms: Serpina1a; serine (or cysteine) peptidase inhibitor, clade A, member 1A; PI1; Aat2; Dom1; Aat-2; Spi1-1; Spi1-3; alpha-1-antitrypsin 1-1; AAT; alpha-1 protease inhibitor 1; alpha-1-antiproteinase; alpha-1-antitrypsin 2; serine (or cysteine) proteinase inhibitor, clade A, member 1a; serine protease inhibitor 1-1; serine protease inhibitor A1a; serpin A1a
• Gene ID: 20700
• mRNA Refseq: NM_001252569
• Protein Refseq: NP_001239498
• UniProt ID: P07758
• Chromosome Location: 12 E; 12 52.98 cM