Recombinant Mouse Mfap5, His-tagged Mfap5-316M

Recombinant Mouse Mfap5, His-tagged


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Recombinant Mouse Mfap5, His-tagged

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Cat.No. : Mfap5-316M
Product Overview : Recombinant Mouse Mfap5 (Accession # AAH25131) Gln29-Leu164, fused with a C-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.
Source : CHO cells
Species : Mouse
Tag : His
Predicted N Terminal : Gln29 predicted, sequencing might be blocked, no result obtained
Form : Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity : Measured by the ability of the immobilized protein to support the adhesion of BUD-8 human fibroblast cells.When 2 x 10^4 cells/well are added to Recombinant Mouse MAGP-2/MFAP5 coated plates (10 µg/mL with 100 µL/well), approximately 45 - 70% will adhere after 1 hour at 37 °C. Optimal dilutions should be determinded by each laboratory for each application.
Molecular Mass : Recombinant Mouse Mfap5, His-tagged has a calculated MW of 16.5 kDa. In SDS-PAGE migrates as 22-30 kDa, reducing conditions.
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : Mfap5 microfibrillar associated protein 5 [ Mus musculus ]
Official Symbol : Mfap5
Synonyms : MFAP5; microfibrillar associated protein 5; microfibrillar-associated protein 5; MFAP-5; microfibril-associated glycoprotein 2; MAGP-2;
Gene ID : 50530
mRNA Refseq : NM_015776
Protein Refseq : NP_056591

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