"Sema5b" Related Products

Recombinant Mouse Sema5b, Fc Chimera

Cat.No.: Sema5b-337M
Product Overview: Recombinant Mouse Sema5b (Accession # AAH52397) was produced in Chinese Hamster Ovary cell line, CHO-derived.
Source: CHO cells
Species: Mouse
Tag: Fc Chimera
Predicted N Terminal: No results obtained, sequencing might be blocked: Gln27 is predicted
Form: Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of human pancreatic cancer cells.When 5 x 10^4 cells/well are added to Recombinant Mouse Semaphorin 5B Fc Chimera coated plates, cell adhesion is enhanced in a dose dependent manner after 1 hour at 37 °C. The ED50 for this effect is typically 1.0 - 4.0 µg/mL.
Molecular Mass: Recombinant Mouse Sema5b, Fc Chimera has a calculated MW of 113.3 kDa (monomer). In SDS-PAGE migrates as 130-150 kDa, reducing conditions.
Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name: Sema5b sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B [ Mus musculus (house mouse) ]
Official Symbol: Sema5b
Synonyms: Sema5b; sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B; SemG; Semag; AI893641; mKIAA1445; semaphorin-5B; sema G; semaphorin 5B; semaphorin-G
Gene ID: 20357
mRNA Refseq: NM_013661
Protein Refseq: NP_038689
MIM:
UniProt ID: Q60519
Chromosome Location: 16; 16 B3

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