Active Recombinant Mouse Cspg5, His-tagged

• Cat.No.: Cspg5-340M
• Product Overview: Recombinant Mouse Cspg5 (Accession # AAH55736) Val31-Gln420, fused with a C-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Val31
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its ability to inhibit Recombinant Human Midkine induced neurite outgrowth in E16-E18 rat embryonic cortical neurons.When 5 x 10^4 neurons per well are added to a plate pre-incubated with serial dilutions of rmNeuroglycan-C and 1 µg/mL of rhMidkine, the neurite outgrowth promotion is significantly inhibited in a dose dependent manner with an effect typically at 0.6 - 1.2 µg/mL.
• Molecular Mass: Recombinant Mouse Cspg5, His-tagged has a calculated MW of 42.1 kDa. In SDS-PAGE migrates as 85-105 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Cspg5 chondroitin sulfate proteoglycan 5 [ Mus musculus ]
• Official Symbol: Cspg5
• Synonyms: CSPG5; chondroitin sulfate proteoglycan 5; neuroglycan C; acidic leucine-rich EGF-like domain-containing brain protein; Ngc; Caleb
• Gene ID: 29873
• mRNA Refseq: NM_001166273
• Protein Refseq: NP_001159745