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Recombinant Human NT5E, His-tagged

Cat.No.: NT5E-343H
Product Overview: Recombinant Human NT5E (Accession # AAH65937) Trp27-Lys547, fused with a C-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.
Source: CHO cells
Species: Human
Tag: His
Predicted N Terminal: Trp27
Form: Supplied as a 0.2 µm filtered solution in Tris, NaCl, CaCl2 and Glycerol.
Bio-activity: Measured by its ability to hydrolyze the 5"-phosphate group from the substrate adenosine-5"-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit .The specific activity is >15,000 pmol/min/µg, as measured under the described conditions.
Molecular Mass: Recombinant Human NT5E, His-tagged has a calculated MW of 59 kDa. In SDS-PAGE migrates as 61-62 kDa, reducing conditions.
Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name: NT5E 5-nucleotidase, ecto (CD73) [ Homo sapiens ]
Official Symbol: NT5E
Synonyms: NT5E; 5-nucleotidase, ecto (CD73); 5 nucleotidase (CD73) , NT5; 5-nucleotidase; CD73; eN; eNT; 5-NT; ecto-5-nucleotidase; Purine 5-Prime-Nucleotidase; NT; NT5; NTE; E5NT;
Gene ID: 4907
mRNA Refseq: NM_001204813
Protein Refseq: NP_001191742
MIM: 129190
UniProt ID: P21589
Chromosome Location: 6q14-q21

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