Recombinant Human NT5E, His-tagged NT5E-343H

Recombinant Human NT5E, His-tagged

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Recombinant Human NT5E, His-tagged

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Cat.No. : NT5E-343H
Product Overview : Recombinant Human NT5E (Accession # AAH65937) Trp27-Lys547, fused with a C-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.
Source : CHO cells
Species : Human
Tag : His
Predicted N Terminal : Trp27
Form : Supplied as a 0.2 µm filtered solution in Tris, NaCl, CaCl2 and Glycerol.
Bio-activity : Measured by its ability to hydrolyze the 5"-phosphate group from the substrate adenosine-5"-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit .The specific activity is >15,000 pmol/min/µg, as measured under the described conditions.
Molecular Mass : Recombinant Human NT5E, His-tagged has a calculated MW of 59 kDa. In SDS-PAGE migrates as 61-62 kDa, reducing conditions.
Purity : >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : NT5E 5-nucleotidase, ecto (CD73) [ Homo sapiens ]
Official Symbol : NT5E
Synonyms : NT5E; 5-nucleotidase, ecto (CD73); 5 nucleotidase (CD73) , NT5; 5-nucleotidase; CD73; eN; eNT; 5-NT; ecto-5-nucleotidase; Purine 5-Prime-Nucleotidase; NT; NT5; NTE; E5NT;
Gene ID : 4907
mRNA Refseq : NM_001204813
Protein Refseq : NP_001191742
MIM : 129190
UniProt ID : P21589
Chromosome Location : 6q14-q21

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