Active Recombinant Mouse Npnt, His-tagged

• Cat.No.: Npnt-346M
• Product Overview: Recombinant Mouse Npnt (Accession # AAH68308) Asp20-Cys561, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Asp20
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of SVEC4-10 mouse vascular endothelial cells.When 5 x 10^4 cells/well are added to rmNephronectin coated plates (10 µg/mL, 100 µL/well), approximately 50 - 80% will adhere after 1 hour at 37 °C.
• Molecular Mass: Recombinant Mouse Npnt, His-tagged has a calculated MW of 60.3 kDa. In SDS-PAGE migrates as 80-95 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Npnt nephronectin [ Mus musculus ]
• Official Symbol: Npnt
• Synonyms: NPNT; nephronectin; preosteoblast EGF-like repeat protein with MAM domain; Nctn; POEM; AA682063; AI314031; 1110009H02Rik
• Gene ID: 114249
• mRNA Refseq: NM_001029836
• Protein Refseq: NP_001025007