Recombinant Mouse Npnt, His-tagged Npnt-346M

Recombinant Mouse Npnt, His-tagged

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Recombinant Mouse Npnt, His-tagged

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Cat.No. : Npnt-346M
Product Overview : Recombinant Mouse Npnt (Accession # AAH68308) Asp20-Cys561, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source : NSO cells
Species : Mouse
Tag : His
Predicted N Terminal : Asp20
Form : Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity : Measured by the ability of the immobilized protein to support the adhesion of SVEC4-10 mouse vascular endothelial cells.When 5 x 10^4 cells/well are added to rmNephronectin coated plates (10 µg/mL, 100 µL/well), approximately 50 - 80% will adhere after 1 hour at 37 °C.
Molecular Mass : Recombinant Mouse Npnt, His-tagged has a calculated MW of 60.3 kDa. In SDS-PAGE migrates as 80-95 kDa, reducing conditions.
Purity : >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : Npnt nephronectin [ Mus musculus ]
Official Symbol : Npnt
Synonyms : NPNT; nephronectin; preosteoblast EGF-like repeat protein with MAM domain; Nctn; POEM; AA682063; AI314031; 1110009H02Rik;
Gene ID : 114249
mRNA Refseq : NM_001029836
Protein Refseq : NP_001025007
MIM :

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