||Recombinant Mouse Itga4&Itgb1 (Accession # AAH68313 (Integrin alpha 4) & P09055 (Integrin beta 1)) was produced in Chinese Hamster Ovary cell line, CHO-derived.
|Predicted N Terminal:
||Tyr34 (Integrin alpha 4) & No results obtained: Gln21 predicted (Integrin beta 1)
||Lyophilized from a 0.2 µm filtered solution in PBS.
||Measured by the ability of the immobilized protein to support the adhesion of CHO Chinese hamster ovary cells transfected with VCAM-1.When 5 x 10^4 cells/well are added to Recombinant Mouse Integrin alpha 4 beta 1 coated plates (10 µg/mL, 100 µL/well), approximately 55-85% will adhere after 1 hour at 37 °C.Optimal dilutions should be determined by each laboratory for each application.
||Recombinant Mouse Itga4&Itgb1 has a calculated MW of 112.9 kDa (Integrin alpha 4) & 86.4 kDa (Integrin beta 1). In SDS-PAGE migrates as 145-160 kDa and 120-140 kDa, reducing donditions.
||>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
||Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).