Recombinant Mouse Itga4&Itgb1 Itga4&Itgb1-347M

Recombinant Mouse Itga4&Itgb1


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Recombinant Mouse Itga4&Itgb1

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Cat.No. : Itga4&Itgb1-347M
Product Overview : Recombinant Mouse Itga4&Itgb1 (Accession # AAH68313 (Integrin alpha 4) & P09055 (Integrin beta 1)) was produced in Chinese Hamster Ovary cell line, CHO-derived.
Source : CHO cells
Species : Mouse
Predicted N Terminal : Tyr34 (Integrin alpha 4) & No results obtained: Gln21 predicted (Integrin beta 1)
Form : Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity : Measured by the ability of the immobilized protein to support the adhesion of CHO Chinese hamster ovary cells transfected with VCAM-1.When 5 x 10^4 cells/well are added to Recombinant Mouse Integrin alpha 4 beta 1 coated plates (10 µg/mL, 100 µL/well), approximately 55-85% will adhere after 1 hour at 37 °C.Optimal dilutions should be determined by each laboratory for each application.
Molecular Mass : Recombinant Mouse Itga4&Itgb1 has a calculated MW of 112.9 kDa (Integrin alpha 4) & 86.4 kDa (Integrin beta 1). In SDS-PAGE migrates as 145-160 kDa and 120-140 kDa, reducing donditions.
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : Itga4 integrin alpha 4 [ Mus musculus ]
Official Symbol : Itga4
Synonyms : ITGA4; integrin alpha 4; integrin alpha-4; integrin alpha-IV; LPAM subunit alpha; VLA-4 subunit alpha; VLA-4 receptor, alpha 4 subunit; CD49 antigen-like family member D; lymphocyte Peyer patch adhesion molecules subunit alpha; CD49D;
Gene ID : 16401
mRNA Refseq : NM_010576
Protein Refseq : NP_034706

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