Active Recombinant Mouse Itga4&Itgb1

• Cat.No.: Itga4&Itgb1-347M
• Product Overview: Recombinant Mouse Itga4&Itgb1 (Accession # AAH68313 (Integrin alpha 4) & P09055 (Integrin beta 1)) was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO cells
• Species: Mouse
• Predicted N Terminal: Tyr34 (Integrin alpha 4) & No results obtained: Gln21 predicted (Integrin beta 1)
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of CHO Chinese hamster ovary cells transfected with VCAM-1.When 5 x 10^4 cells/well are added to Recombinant Mouse Integrin alpha 4 beta 1 coated plates (10 µg/mL, 100 µL/well), approximately 55-85% will adhere after 1 hour at 37 °C.Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Mouse Itga4&Itgb1 has a calculated MW of 112.9 kDa (Integrin alpha 4) & 86.4 kDa (Integrin beta 1). In SDS-PAGE migrates as 145-160 kDa and 120-140 kDa, reducing donditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Itga4 integrin alpha 4 [ Mus musculus ]
• Official Symbol: Itga4
• Synonyms: ITGA4; integrin alpha 4; integrin alpha-4; integrin alpha-IV; LPAM subunit alpha; VLA-4 subunit alpha; VLA-4 receptor, alpha 4 subunit; CD49 antigen-like family member D; lymphocyte Peyer patch adhesion molecules subunit alpha; CD49D
• Gene ID: 16401
• mRNA Refseq: NM_010576
• Protein Refseq: NP_034706