Recombinant Mouse Ecm1 protein, His-tagged
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|Product Overview :||Recombinant Mouse Ecm1(AAla20-Glu559) fused with His tag at C-terminal was expressed in NS0.|
Extracellular matrix protein-1 (ECM-1, ECM-1a) is an 85 kDa, secreted glycoprotein important in connective tissue organization. Of identified splice variants, the 559 amino acid (aa) form, ECM-1a is most widely expressed, with highest ex
|Predicted N Terminal :||Ala20|
|Form :||Lyophilized from a 0.2 μm filtered solution in PBS.|
Measured by the ability of the immobilized protein to support the adhesion of BUD‑8 human fibroblast cells.
When 2.5 x 104 cells/well are added to rmECM-1 coated plates (5 µg/mL, 100 µL/well), approximately 55‑85% will adhere after 60 minutes at 37° C.
Optimal dilutions should be determined by each laboratory for each application.
|Molecular Mass :||
Predicted Molecular Mass: 61.8 (monomer) kDa;
SDS-PAGE: 80-90 kDa, redcucing conditions.
|Protein length :||Ala20-Glu559|
|Endotoxin :||<0.10 EU per 1 μg of the protein by the LAL method.|
|Purity :||>95%, by SDS-PAGE under reducing conditions and visualized by silver stain|
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Reconstitution :||Reconstitute at 300 μg/mL in PBS.|
|Shipping :||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Gene Name :||Ecm1 extracellular matrix protein 1 [ Mus musculus ]|
|Official Symbol :||Ecm1|
|Synonyms :||ECM1; extracellular matrix protein 1; secretory component p85; p85; AI663821;|
|Gene ID :||13601|
|mRNA Refseq :||NM_001252653|
|Protein Refseq :||NP_001239582|
|UniProt ID :||Q61508|
|Chromosome Location :||3 F2.1; 3 40.91 cM|
|Function :||enzyme binding; interleukin-2 receptor binding; laminin binding; protease binding; protein C-terminus binding; signal transducer activity;|
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