Recombinant Cotton Rat Tnf, Met-tagged Tnf-391C

Recombinant Cotton Rat Tnf, Met-tagged

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Recombinant Cotton Rat Tnf, Met-tagged

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Cat.No. : Tnf-391C
Product Overview : Recombinant Cotton Rat Tnf (Accession # AAL18818) Leu1-Leu156, fused with an N-terminal Met, was produced in E. coli-derived.
Source : E. coli
Species : Cotton Rat
Predicted N Terminal : Met
Form : Lyophilized from a 0.2 µm filtered solution in NaH2PO4, NaCl and EDTA with BSA as a carrier protein.
Bio-activity : Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221.The ED50 for this effect is typically 0.03-0.12 ng/mL.
Molecular Mass : Recombinant Cotton Rat Tnf, Met-tagged has a calculated MW of 17.5 kDa.
Purity : >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution : Reconstitute at 25 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Gene Name : Tnf tumor necrosis factor [ Rattus norvegicus (Norway rat) ]
Official Symbol : Tnf
Synonyms : Tnf; tumor necrosis factor; tumor necrosis factor; cachectin; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor ligand superfamily member 2
Gene ID : 24835
mRNA Refseq : NM_012675
Protein Refseq : NP_036807
MIM :
UniProt ID : P16599
Chromosome Location : 20p12

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