Active Recombinant Mouse Treml1, His-tagged

• Cat.No.: Treml1-398M
• Product Overview: Recombinant Mouse Treml1 (Accession # AAL85488) Gly18-Cys178, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Gly18
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of thrombin-activated human platelets.When 5 x 10^6 cells per well are added to rmTLT-1 coated plates (2 µg/mL, 100 µL/well), approximately 50% - 70% will adhere after 1 hour at 37 °C. Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Mouse Treml1, His-tagged has a calculated MW of 18.3 kDa. In SDS-PAGE migrates as 21-23 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Publication:
  Platelet-derived TLT-1 promotes tumor progression by suppressing CD8+ T cells (2023)

Gene Information

• Gene Name: Treml1 triggering receptor expressed on myeloid cells-like 1 [ Mus musculus ]
• Official Symbol: Treml1
• Synonyms: TREML1; triggering receptor expressed on myeloid cells-like 1; trem-like transcript 1 protein; triggering receptor expressed on myeloid cells-like 1c; triggering receptor expressed on myeloid cells-like 1d; triggering receptor expressed on myeloid cells-like 1e; triggering receptor expressed on myeloid cells-like protein 1; TLT-1; 5430401J17Rik
• Gene ID: 71326
• mRNA Refseq: NM_027763
• Protein Refseq: NP_082039