Active Recombinant Mouse Fgfr2, Fc Chimera

• Cat.No.: Fgfr2-427M
• Product Overview: Recombinant Mouse Fgfr2 (Accession # ABL89196) was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: Fc Chimera
• Predicted N Terminal: Arg22, Ser24, and Leu27
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its binding ability in a functional ELISA.200 ng/mL of biotinylated rmFGF1 is mixed with serially diluted rmFGF R2 alpha (IIIb)/Fc. Following incubation, the FGF R2 alpha (IIIb)/Fc - FGF1/biotin complex is captured on a Streptavidin coated plate. Bound FGF R2 alpha (IIIb)/Fc is measured using HRP labeled Gt x Human IgG Fc. The concentration of rmFGF R2 alpha (IIIb)/Fc that produces 50% of the optimal binding was found to be approximately 150 - 600 ng/mL.
• Molecular Mass: Recombinant Mouse Fgfr2, Fc Chimera has a calculated MW of 66.2 kDa (monomer). In SDS-PAGE migrates as 105-120 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Fgfr2 fibroblast growth factor receptor 2 [ Mus musculus ]
• Official Symbol: Fgfr2
• Synonyms: FGFR2; fibroblast growth factor receptor 2; keratinocyte growth factor receptor; Bek; svs; KGFR; Fgfr7; Fgfr-2; Fgfr-7; KGFRTr; AU043015; AW556123; MGC102519
• Gene ID: 14183
• mRNA Refseq: NM_010207
• Protein Refseq: NP_034337