|Product Overview:||Recombinant Human GFP(Ser2-Lys238)/SNAP25B(Asn93-Gly206)/VAMP-2(Ser2-Lys94) fused with His tag at N-terminal was expressed in E. coli.|
|Predicted N Terminal:||Val (of MVKSADI)|
|Form:||Supplied as a 0.2 μm filtered solution in Tris, NaCl, DTT and Tween®.|
|Bio-activity:||Measured by cleavage by botulinum and tetanus toxin light chains. >50% of 1 μg can be cleaved by 4 ng of toxin light chain.|
|Molecular Mass:||Predicted Molecular Mass: 52 kDa;
SDS-PAGE: 54-58 kDa, reducing conditions.
|Endotoxin:||<1.0 EU per 1 μg of the protein by the LAL method.|
|Purity:||>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane|
|Storage:||Avoid repeated freeze-thaw cycles.
6 months from date of receipt, -70 centigrade as supplied.
3 months, -70 centigrade under sterile conditions after opening.