Recombinant Human GFP/SNAP25B/VAMP-2 protein, His-tagged

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Recombinant Human GFP/SNAP25B/VAMP-2 protein, His-tagged

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Cat.No. : GFP-430
Product Overview : Recombinant Human GFP(Ser2-Lys238)/SNAP25B(Asn93-Gly206)/VAMP-2(Ser2-Lys94) fused with His tag at N-terminal was expressed in E. coli.
Source : E. coli
Species : Human
Tag : His
Predicted N Terminal : Val (of MVKSADI)
Form : Supplied as a 0.2 μm filtered solution in Tris, NaCl, DTT and Tween®.
Bio-activity : Measured by cleavage by botulinum and tetanus toxin light chains. >50% of 1 μg can be cleaved by 4 ng of toxin light chain.
Molecular Mass : Predicted Molecular Mass: 52 kDa;
SDS-PAGE: 54-58 kDa, reducing conditions.
Endotoxin : <1.0 EU per 1 μg of the protein by the LAL method.
Purity : >85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane
Storage : Avoid repeated freeze-thaw cycles.
6 months from date of receipt, -70 centigrade as supplied.
3 months, -70 centigrade under sterile conditions after opening.

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