Recombinant Human GFP/SNAP25B/VAMP-2 protein, His-tagged
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|Product Overview :||Recombinant Human GFP(Ser2-Lys238)/SNAP25B(Asn93-Gly206)/VAMP-2(Ser2-Lys94) fused with His tag at N-terminal was expressed in E. coli.|
|Source :||E. coli|
|Predicted N Terminal :||Val (of MVKSADI)|
|Form :||Supplied as a 0.2 μm filtered solution in Tris, NaCl, DTT and Tween®.|
|Bio-activity :||Measured by cleavage by botulinum and tetanus toxin light chains. >50% of 1 μg can be cleaved by 4 ng of toxin light chain.|
|Molecular Mass :||Predicted Molecular Mass: 52 kDa;
SDS-PAGE: 54-58 kDa, reducing conditions.
|Endotoxin :||<1.0 EU per 1 μg of the protein by the LAL method.|
|Purity :||>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane|
|Storage :||Avoid repeated freeze-thaw cycles.
6 months from date of receipt, -70 centigrade as supplied.
3 months, -70 centigrade under sterile conditions after opening.
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