Active Recombinant Human GFP/SNAP25B/VAMP-2 protein, His-tagged

• Cat.No.: GFP-430
• Product Overview: Recombinant Human GFP(Ser2-Lys238)/SNAP25B(Asn93-Gly206)/VAMP-2(Ser2-Lys94) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 2-238;93-206;2-94 a.a.
• Predicted N Terminal: Val (of MVKSADI)
• Form: Supplied as a 0.2 μm filtered solution in Tris, NaCl, DTT and Tween®.
• Bio-activity: Measured by cleavage by botulinum and tetanus toxin light chains. >50% of 1 μg can be cleaved by 4 ng of toxin light chain.
• Molecular Mass: Predicted Molecular Mass: 52 kDa;
  SDS-PAGE: 54-58 kDa, reducing conditions.
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane
• Storage: Avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -70 centigrade as supplied.
  3 months, -70 centigrade under sterile conditions after opening.