Recombinant Horse IFNG protein IFNG-440E

Recombinant Horse IFNG protein

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Recombinant Horse IFNG protein

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Cat.No. : IFNG-440E
Product Overview : Recombinant Horse IFNG protein was expressed in Escherichia coli.
Description : This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections.
Source : E.coli
Species : Horse
Form : Lyophilized from a 0.2μm filtered concentrated solution in 2 × PBS, pH 7.4, with 5 % trehalose.
Bio-activity : Fully biologically active when compared to standard. The ED50 as determined by an anti-viral assay using human HeLa cells infected with encephalomyocarditis (EMC) virus is less than 10.0 ng/ml, corresponding to a specific activity of > 1.0 × 10⁵ IU/mg.
Molecular Mass : Approximately 16.7 kDa, a single non-glycosylated polypeptide chain containing 143 amino acids.
Protein length : 143
AA Sequence : QAAFFKEIENLKEYF NASNPDVGDGGPLFL DILKNWKEDSDKKII QSQIVSFYFKLFENL KDNQVIQKSMDTIKE DLFVKFFNSSTSKLE DFQKLIQIPVNDLKV QRKAISELIKVMNDL SPKANLRKRKRSQNP FRGRRALQ
Endotoxin : Less than 0.1 EU/µg of rEqIFN-γ as determined by LAL method.
Purity : >97% by SDS-PAGE and HPLC analysis.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.
SDS-PAGE of IFNG-440E :

Gene Name : IFNG
Official Symbol : IFNG
Synonyms : IFNG; interferon, gamma; interferon gamma; IFN-gamma;
Gene ID : 100034181
mRNA Refseq : NM_001081949
Protein Refseq : NP_001075418
UniProt ID : P42160

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