Recombinant Human Carbonic Anhydrase II
Cat.No. : | CA2-144H |
Product Overview : | Recombinant human CA2 was expressed in E.coli and purified by using conventional chromatography, 29.2 kDa (260aa) |
- Specification
- Gene Information
- Related Products
Cat. No. : | CA2-144H |
Description : | Carbonic anhydrase 2 (CA2) is one of fourteen forms of humanα carbonic anhydrases. Carbonic anhydrase catalyzes reversible hydration of carbon dioxide. CA2 is a cytosolic enzyme with the highest activity among all known CAs. Mutations in the CA2 gene result in the CA2 deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification. |
Purity : | > 95% by SDS - PAGE |
Sequences of amino acids : | MSHHWGYGKH NGPEHWHKDF PIAKGERQSP VDIDTHTAKY DPSLKPLSVS YDQATSLRIL NNGHAFNVEF DDSQDKAVLK GGPLDGTYRL IQFHFHWGSL DGQGSEHTVD KKKYAAELHL VHWNTKYGDF GKAVQQPDGL AVLGIFLKVG SAKPGLQKVV DVLDSIKTKG KSADFTNFDP RGLLPESLDY WTYPGSLTTP PLLECVTWIV LKEPISVSSE QVLKFRKLNF NGEGEPEELM VDNWRPAQPL KNRQIKASFK |
Concentration : | 1 mg/ml (determined by Bradford assay) |
Form : | Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 50mM NaCl, 10% glycerol |
Biological activity : | Specific activity is 50-70 nmoles/min/ug and was obtained by measuring the increase in the amount of p-nitrophenol by its esterase activity. Specific activity is defined as the amount of p-nitrophenol that 1ug of enzyme can reduce at 25℃ for 1 minute. |
Activity Assay : | 1. Prepare assay buffer into a suitable container: The final concentrations are 12.5 mM Tris, 75 mM NaCl, pH 7.5. 2. Dilute 4-Nitrophenyl-Acetate(4-NPA) to 2 mM in Assay Buffer. 3. Dilute recombinant CA2 protein with various concentrations (1ug, 2ug, 5ug) in 50 ul assay buffer. 4. Load 50 μL of 2mM 4-NPA into diluted recombinant CA2 solutions, and start the reaction by adding 50 μL of 2 mM Substrate. 5. Read at a wavelength of 405 nm (bottom read) in kinetic mode for 5 minutes. |
Storage : | Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |
Gene Name : | CA2 carbonic anhydrase II [ Homo sapiens ] |
Synonyms : | CA2; carbonic anhydrase II; CAII; Car2; CA-II; CA2; carbonic anhydrase B; carbonic dehydratase; carbonate dehydratase II; EC 4.2.1.1; CAC; Carbonic anhydrase C |
Gene ID : | 760 |
mRNA Refseq : | NM_000067 |
Protein Refseq : | NP_000058 |
MIM : | 611492 |
UniProt ID : | P00918 |
Chromosome Location : | 8q22 |
Pathway : | Nitrogen metabolism |
Function : | carbonate dehydratase activity; lyase activity; metal ion binding; zinc ion binding |
Products Types
◆ Recombinant Protein | ||
Car2-765M | Recombinant Mouse Car2 Protein, MYC/DDK-tagged | +Inquiry |
CA2-201H | Recombinant Human CA2 Protein, MYC/DDK-tagged, C13 and N15-labeled | +Inquiry |
CA2-479H | Recombinant Human CA2 Protein, His (Fc)-Avi-tagged | +Inquiry |
CA2-0237H | Active Recombinant Human CA2 Protein | +Inquiry |
CA2-716R | Recombinant Rat CA2 Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Native Protein | ||
CA2-32S | Native Sheep Carbonic Anhydrase II (CA2) Protein | +Inquiry |
CA2-31M | Native Mouse Carbonic Anhydrase II (CA2) Protein | +Inquiry |
CA2-34R | Native Rabbit Carbonic Anhydrase II (CA2) Protein | +Inquiry |
◆ Lysates | ||
CA2-7915HCL | Recombinant Human CA2 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionCA2 deficiency is a rare autosomal recessive disorder with metabolic acidosis and osteopetrosis.
Acetazolamide is a known inhibitor, used clinically for glaucoma and altitude sickness.
Some mutations can lead to CA2 deficiency, affecting acid-base balance and leading to metabolic acidosis.
CA2 is one of the most efficient and fastest enzymes, displaying higher activity than many other isoforms.
CA2 accelerates the reversible conversion of CO2 and water to bicarbonate and protons, aiding in pH regulation.
CA2 plays a crucial role in CO2 transport and acid-base balance in various tissues.
CA2 is ubiquitously expressed but is particularly abundant in red blood cells and certain epithelial tissues.
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