Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Recombinant Human Carbonic Anhydrase II

Cat.No. : CA2-144H
Product Overview : Recombinant human CA2 was expressed in E.coli and purified by using conventional chromatography, 29.2 kDa (260aa)
  • Specification
  • Gene Information
  • Related Products
Cat. No. : CA2-144H
Description : Carbonic anhydrase 2 (CA2) is one of fourteen forms of humanα carbonic anhydrases. Carbonic anhydrase catalyzes reversible hydration of carbon dioxide. CA2 is a cytosolic enzyme with the highest activity among all known CAs. Mutations in the CA2 gene result in the CA2 deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification.
Purity : > 95% by SDS - PAGE
Sequences of amino acids : MSHHWGYGKH NGPEHWHKDF PIAKGERQSP VDIDTHTAKY DPSLKPLSVS YDQATSLRIL NNGHAFNVEF DDSQDKAVLK GGPLDGTYRL IQFHFHWGSL DGQGSEHTVD KKKYAAELHL VHWNTKYGDF GKAVQQPDGL AVLGIFLKVG SAKPGLQKVV DVLDSIKTKG KSADFTNFDP RGLLPESLDY WTYPGSLTTP PLLECVTWIV LKEPISVSSE QVLKFRKLNF NGEGEPEELM VDNWRPAQPL KNRQIKASFK
Concentration : 1 mg/ml (determined by Bradford assay)
Form : Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 50mM NaCl, 10% glycerol
Biological activity : Specific activity is 50-70 nmoles/min/ug and was obtained by measuring the increase in the amount of p-nitrophenol by its esterase activity. Specific activity is defined as the amount of p-nitrophenol that 1ug of enzyme can reduce at 25℃ for 1 minute.
Activity Assay : 1. Prepare assay buffer into a suitable container: The final concentrations are 12.5 mM Tris, 75 mM NaCl, pH 7.5. 2. Dilute 4-Nitrophenyl-Acetate(4-NPA) to 2 mM in Assay Buffer. 3. Dilute recombinant CA2 protein with various concentrations (1ug, 2ug, 5ug) in 50 ul assay buffer. 4. Load 50 μL of 2mM 4-NPA into diluted recombinant CA2 solutions, and start the reaction by adding 50 μL of 2 mM Substrate. 5. Read at a wavelength of 405 nm (bottom read) in kinetic mode for 5 minutes.
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name : CA2 carbonic anhydrase II [ Homo sapiens ]
Synonyms : CA2; carbonic anhydrase II; CAII; Car2; CA-II; CA2; carbonic anhydrase B; carbonic dehydratase; carbonate dehydratase II; EC 4.2.1.1; CAC; Carbonic anhydrase C
Gene ID : 760
mRNA Refseq : NM_000067
Protein Refseq : NP_000058
MIM : 611492
UniProt ID : P00918
Chromosome Location : 8q22
Pathway : Nitrogen metabolism
Function : carbonate dehydratase activity; lyase activity; metal ion binding; zinc ion binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (7)

Ask a question
Is CA2 involved in any particular pathological conditions? 07/05/2022

CA2 deficiency is a rare autosomal recessive disorder with metabolic acidosis and osteopetrosis.

What are the known inhibitors of CA2 and their clinical implications? 02/23/2022

Acetazolamide is a known inhibitor, used clinically for glaucoma and altitude sickness.

How do mutations in the CA2 gene influence enzyme activity or stability? 06/13/2021

Some mutations can lead to CA2 deficiency, affecting acid-base balance and leading to metabolic acidosis.

How does CA2's enzymatic activity compare to other carbonic anhydrase isoforms? 07/28/2019

CA2 is one of the most efficient and fastest enzymes, displaying higher activity than many other isoforms.

What role does CA2 play in the physiological regulation of pH? 07/23/2019

CA2 accelerates the reversible conversion of CO2 and water to bicarbonate and protons, aiding in pH regulation.

Are there specific cellular pathways where CA2 plays a significant role? 11/14/2018

CA2 plays a crucial role in CO2 transport and acid-base balance in various tissues.

How is CA2 expression distributed across different human tissues? 07/04/2018

CA2 is ubiquitously expressed but is particularly abundant in red blood cells and certain epithelial tissues.

Customer Reviews (3)

Write a review
Reviews
05/01/2022

    Efficient sample processing, a time-saving boon for researchers.

    08/30/2021

      Professional expertise, elevates our protein research projects.

      06/08/2021

        Invaluable assistance for intricate protein experiments.

        Ask a Question for All CA2 Products

        Required fields are marked with *

        My Review for All CA2 Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends