Recombinant Mouse Hrg, His-tagged


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Recombinant Mouse Hrg, His-tagged

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Cat.No. : Hrg-452M
Product Overview : Recombinant Mouse Hrg (Accession # BAB33094) Leu19-Lys525, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source : NSO cells
Species : Mouse
Tag : His
Predicted N Terminal : Leu19
Form : Lyophilized from a 0.2 µm filtered solution in MES and NaCl.
Bio-activity : Measured by its ability to support the adhesion of MOLT-4 human acute lymphoblastic leukemia cells. Lamb-Wharton, R.J. and W.T. Morgen (1993) Cell Immunol. 152:544.When 1 x 10^5 cells/well are added to Recombinant Mouse HPRG coated plates, adhesion is induced in a dose-dependent manner in the presence of Concanvalin A. The ED50 for this effect is typically 0.6-3 µg/mL.
Molecular Mass : Recombinant Mouse Hrg, His-tagged has a calculated MW of 58.5 kDa. In SDS-PAGE migrates as 80-85 kDa, reducing conditions.
Purity : >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : Hrg histidine-rich glycoprotein [ Mus musculus ]
Official Symbol : Hrg
Synonyms : HRG; histidine-rich glycoprotein; histidine-proline-rich glycoprotein; Hprg; Hrgp; D16jh2; D18020; AI265597; AW413091;
Gene ID : 94175
mRNA Refseq : NM_053176
Protein Refseq : NP_444406

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