Active Recombinant Mouse Hrg, His-tagged

• Cat.No.: Hrg-452M
• Product Overview: Recombinant Mouse Hrg (Accession # BAB33094) Leu19-Lys525, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Leu19
• Form: Lyophilized from a 0.2 µm filtered solution in MES and NaCl.
• Bio-activity: Measured by its ability to support the adhesion of MOLT-4 human acute lymphoblastic leukemia cells. Lamb-Wharton, R.J. and W.T. Morgen (1993) Cell Immunol. 152:544.When 1 x 10^5 cells/well are added to Recombinant Mouse HPRG coated plates, adhesion is induced in a dose-dependent manner in the presence of Concanvalin A. The ED50 for this effect is typically 0.6-3 µg/mL.
• Molecular Mass: Recombinant Mouse Hrg, His-tagged has a calculated MW of 58.5 kDa. In SDS-PAGE migrates as 80-85 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Hrg histidine-rich glycoprotein [ Mus musculus ]
• Official Symbol: Hrg
• Synonyms: HRG; histidine-rich glycoprotein; histidine-proline-rich glycoprotein; Hprg; Hrgp; D16jh2; D18020; AI265597; AW413091
• Gene ID: 94175
• mRNA Refseq: NM_053176
• Protein Refseq: NP_444406