Recombinant Human IGFBP3, Met-tagged IGFBP3-489H

Recombinant Human IGFBP3, Met-tagged

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Recombinant Human IGFBP3, Met-tagged

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Cat.No. : IGFBP3-489H
Product Overview : Recombinant Human IGFBP3 (Accession # CAA46087) Gly28-Lys291, fused with an N-terminal Met, was produced in Mouse myeloma cell line, NS0-derived.
Source : NSO cells
Species : Human
Predicted N Terminal : Met
Form : Lyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA.
Bio-activity : Measured by its ability to inhibit the biological activity of IGF-I or IGF-II on MCF-7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083.The ED50 for this effect is typically 0.05 - 0.15 µg/mL in the presence of 14 ng/mL rhIGF-II.
Molecular Mass : Recombinant Human IGFBP3, Met-tagged has a calculated MW of 29 kDa. In SDS-PAGE migrates as 41 kDa, reducing conditions.
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : IGFBP3 insulin-like growth factor binding protein 3 [ Homo sapiens ]
Official Symbol : IGFBP3
Synonyms : IGFBP3; insulin-like growth factor binding protein 3; insulin-like growth factor-binding protein 3; acid stable subunit of the 140 K IGF complex; binding protein 29; binding protein 53; BP 53; growth hormone dependent binding protein; IBP3; IGF binding protein 3; IBP-3; IGFBP-3; IGF-binding protein 3; growth hormone-dependent binding protein; BP-53;
Gene ID : 3486
mRNA Refseq : NM_000598
Protein Refseq : NP_000589
MIM : 146732
UniProt ID : P17936
Chromosome Location : 7p13-p12

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