Active Recombinant Mouse Vcam1, Fc Chimera

• Cat.No.: Vcam1-491M
• Product Overview: Recombinant Mouse Vcam1 (Accession # CAA47989) was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: Fc Chimera
• Predicted N Terminal: Met
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells.When 5 x 10^4 cells/well are added to mouse VCAM-1 coated plates (10 µg/mL with 100 µL/well), >85% will adhere after 1 hour incubation at room temperature. Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Mouse Vcam1, Fc Chimera has a calculated MW of 101.9 kDa (monomer). In SDS-PAGE migrates as 120 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Vcam1 vascular cell adhesion molecule 1 [ Mus musculus ]
• Official Symbol: Vcam1
• Synonyms: VCAM1; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; V-CAM 1; CD106; Vcam-1
• Gene ID: 22329
• mRNA Refseq: NM_011693
• Protein Refseq: NP_035823