Active Recombinant Human CDH5 protein, His-tagged

• Cat.No.: CDH5-501H
• Product Overview: Recombinant Human CDH5(Asp48-Gln599) fused with His tag at C-terminal was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Predicted N Terminal: Asp48
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of BCE C/D‑1b bovine corneal endothelial cells. The ED50 for this effect is typically 20-100 ng/mL.Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Predicted Molecular Mass: 63 kDa;SDS-PAGE: 80-94 kDa, reducing conditions
• Protein length: Asp48-Gln599
• Endotoxin: <0.10 EU per 1 mug of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE with silver staining
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 250 μg/mL in sterile PBS.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Gene Information

• Gene Name: CDH5 cadherin 5, type 2 (vascular endothelium) [ Homo sapiens ]
• Official Symbol: CDH5
• Synonyms: CDH5; cadherin 5, type 2 (vascular endothelium); cadherin 5, type 2, VE cadherin (vascular epithelium); cadherin-5; 7B4; CD144; VE cadherin; 7B4 antigen; VE-cadherin; cd144 antigen; endothelial-specific cadherin; vascular endothelial cadherin; cadherin 5, type 2, VE-cadherin (vascular epithelium); FLJ17376
• Gene ID: 1003
• mRNA Refseq: NM_001795
• Protein Refseq: NP_001786
• MIM: 601120
• UniProt ID: P33151
• Chromosome Location: 16q22.1
• Pathway: Adherens junctions interactions, organism-specific biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Cell junction organization, organism-specific biosystem; Cell-Cell communication, organism-specific biosystem; Cell-cell junction organization, organism-specific biosystem; Leukocyte transendothelial migration, organism-specific biosystem
• Function: RPTP-like protein binding; beta-catenin binding; calcium ion binding; ion channel binding; protein binding; protein phosphatase binding; receptor binding