Recombinant Canine TNF, Met-tagged

Cat.No.: TNF-504C
Product Overview: Recombinant Canine TNF (Accession # CAA64403) Val77-Leu233, fused with an N-terminal Met, was produced in E. coli-derived.
Source: E. coli
Species: Canine
Predicted N Terminal: Met
Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
Bio-activity: Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221.The ED50 for this effect is typically 0.6-3 ng/mL.
Molecular Mass: Recombinant Canine TNF, Met-tagged has a calculated MW of 17.3 kDa.
Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Gene Name: NF tumor necrosis factor [ Canis lupus familiaris (dog) ]
Official Symbol: TNF
Synonyms: TNF; tumor necrosis factor; TNFA; cTNF; tumor necrosis factor; TNF-a; TNF-alpha; cachectin; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor ligand superfamily member 2; tumour necrosis factor
Gene ID: 403922
mRNA Refseq: NM_001003244
Protein Refseq: NP_001003244
MIM:
UniProt ID: P51742
Chromosome Location: 12

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