Active Recombinant Mouse Twsg1, His-tagged

• Cat.No.: Twsg1-514M
• Product Overview: Recombinant Mouse Twsg1 (Accession # CAC05586) Cys25-Phe222, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Cys25
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured by its ability to inhibit BMP-6-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells.The ED50 for this effect is typically 1 - 4 µg/mL in the presence of 150 ng/mL of Recombinant Human BMP-6.
• Molecular Mass: Recombinant Mouse Twsg1, His-tagged has a calculated MW of 23.5 kDa. In SDS-PAGE migrates as 25-35 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: Twsg1 twisted gastrulation homolog 1 (Drosophila) [ Mus musculus ]
• Official Symbol: Twsg1
• Synonyms: TWSG1; twisted gastrulation homolog 1 (Drosophila); twisted gastrulation protein homolog 1; Tsg; Twg; AW552143; D17Ertd403e; 1810013J15Rik; 9030422N06Rik
• Gene ID: 65960
• mRNA Refseq: NM_023053
• Protein Refseq: NP_075540