Recombinant Mouse Twsg1, His-tagged Twsg1-514M

Recombinant Mouse Twsg1, His-tagged


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Recombinant Mouse Twsg1, His-tagged

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Cat.No. : Twsg1-514M
Product Overview : Recombinant Mouse Twsg1 (Accession # CAC05586) Cys25-Phe222, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source : NSO cells
Species : Mouse
Tag : His
Predicted N Terminal : Cys25
Form : Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
Bio-activity : Measured by its ability to inhibit BMP-6-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells.The ED50 for this effect is typically 1 - 4 µg/mL in the presence of 150 ng/mL of Recombinant Human BMP-6.
Molecular Mass : Recombinant Mouse Twsg1, His-tagged has a calculated MW of 23.5 kDa. In SDS-PAGE migrates as 25-35 kDa, reducing conditions.
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution : Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Gene Name : Twsg1 twisted gastrulation homolog 1 (Drosophila) [ Mus musculus ]
Official Symbol : Twsg1
Synonyms : TWSG1; twisted gastrulation homolog 1 (Drosophila); twisted gastrulation protein homolog 1; Tsg; Twg; AW552143; D17Ertd403e; 1810013J15Rik; 9030422N06Rik;
Gene ID : 65960
mRNA Refseq : NM_023053
Protein Refseq : NP_075540

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