||Recombinant RSV Src (Accession # M11753) , fused with an N-terminal GST tag, was produced in baculovirus in Sf 9 insect cells.
||Sf 9 insect cells
||Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EDTA, 0.1 mM EGTA, 0.1 mM PMSF, and 25% glycerol.
||The specific activity of Src was determined to be 116 nmol/min/mg using a synthetic peptide substrate (KVEKIGEGTYGVVYK)
||Recombinant RSV Src, GST-tagged has a calculated MW of 85 kDa.
||The purity was determined to be >90% by densitometry
||Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).