Active Recombinant Human SUMO1

• Cat.No.: SUMO1-560H
• Product Overview: Recombinant Human SUMO1 (Accession # NM_003352) , fused with a C-terminal Aminoluciferin (AML), was produced in E. coli-derived.

Specification

• Source: E. coli
• Species: Human
• Form: X mg/ml (X µM) in 50 mM HEPES pH 7.5, 100 mM NaCl
• Bio-activity: Recombinant Human SUMO1-Aminoluciferin (AML) is ideal for use as a SUMO-specific isopeptidase enzyme substrate. Isopeptidase activity liberates luciferin from Recombinant Human SUMO1-Aminoluciferin (AML). ATP and luciferase are then added to produce a luminescent signal proportional to SUMO-specific isopeptidase activity. Optimal luminescence at pH 7.5 can be monitored using all wavelengths with a 500 ms integration time. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human SUMO1-Aminoluciferin (AML) concentration of 0.1-1 µM.
• Molecular Mass: Recombinant Human SUMO1 has a calculated MW of 11 kDa.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: SUMO1 SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: SUMO1
• Synonyms: SUMO1; SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae); SMT3 suppressor of mif two 3 homolog 1 (yeast) , ubiquitin like 1 (sentrin) , UBL1; small ubiquitin-related modifier 1; GMP1; OFC10; PIC1; SMT3C; SMT3H3; SUMO 1; sentrin; SMT3 homolog 3; GAP modifying protein 1; ubiquitin-like protein UBL1; ubiquitin-like protein SMT3C; ubiquitin-homology domain protein PIC1; DAP1; SMT3; UBL1; SENP2
• Gene ID: 7341
• mRNA Refseq: NM_001005781
• Protein Refseq: NP_001005781
• MIM: 601912
• UniProt ID: P63165
• Chromosome Location: 2q33