Active Recombinant Human COL3A1, His-tagged

• Cat.No.: COL3A1-604H
• Product Overview: Recombinant Human COL3A1 (Accession # NP_000081) Thr1097-Leu1466, fused with an N-terminal human CD33 signal sequence and 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: His
• Form: Supplied as a 0.2 µm filtered solution in Sodium Acetate and NaCl.
• Bio-activity: Measured by the cleavage of its C-terminal propeptide by Recombinant Human BMP-1/PCP .>50% of full-length Pro-Collagen III alpha 1 is cleaved by recombinant human BMP-1, as measured under the described conditions.
• Molecular Mass: Recombinant Human COL3A1, His-tagged has a calculated MW of 40 kDa. In SDS-PAGE migrates as 41-46 kDa, reducing conditions.
• Purity: >75%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: COL3A1 collagen, type III, alpha 1 [ Homo sapiens ]
• Official Symbol: COL3A1
• Synonyms: COL3A1; collagen, type III, alpha 1; EDS4A, Ehlers Danlos syndrome type IV, autosomal dominant; collagen alpha-1(III) chain; collagen, fetal; alpha1 (III) collagen; Ehlers-Danlos syndrome type IV, autosomal dominant; EDS4A; FLJ34534
• Gene ID: 1281
• mRNA Refseq: NM_000090
• Protein Refseq: NP_000081
• MIM: 120180
• UniProt ID: P02461
• Chromosome Location: 2q32.2